Modulation of Nb2 cell mitogenesis by peripheral benzodiazepine ligands

Persistent Link:
http://hdl.handle.net/10150/277200
Title:
Modulation of Nb2 cell mitogenesis by peripheral benzodiazepine ligands
Author:
Gerrish, Kevin Edward, 1965-
Issue Date:
1989
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
In this study, we investigated the effects of the peripheral benzodiazepine ligands, Ro5-4864 (putative antagonist) and PK 11195 (putative antagonist) on prolactin stimulated mitogenesis in Nb2 cells. Ro5-4864 and PK 11195 at 10⁻⁹ M maximally enhanced prolactin stimulated mitogenesis. At 10⁻⁶ M Ro5-4864 inhibited prolactin stimulated mitogenesis. Clonazepam, a ligand for the central benzodiazepine receptor had no effect on mitogenesis. Interaction studies were undertaken to determine if Ro5-4864 and PK 11195 act on the same site. The ability of each ligand to enhance the mitogenic action of prolactin was blocked by a 10⁻⁶ M concentration of the other ligand. Finally, simultaneous addition of 10⁻⁹ M of the ligands resulted in no additive effect over each ligand alone. These data show that peripheral benzodiazepine ligands modulate prolactin-stimulated mitogenesis and suggests they interact at the same binding site.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Benzodiazepines.; Cell division.; Cell culture.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Pharmacology and Toxicology
Degree Grantor:
University of Arizona
Advisor:
Laird, Hugh E.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleModulation of Nb2 cell mitogenesis by peripheral benzodiazepine ligandsen_US
dc.creatorGerrish, Kevin Edward, 1965-en_US
dc.contributor.authorGerrish, Kevin Edward, 1965-en_US
dc.date.issued1989en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractIn this study, we investigated the effects of the peripheral benzodiazepine ligands, Ro5-4864 (putative antagonist) and PK 11195 (putative antagonist) on prolactin stimulated mitogenesis in Nb2 cells. Ro5-4864 and PK 11195 at 10⁻⁹ M maximally enhanced prolactin stimulated mitogenesis. At 10⁻⁶ M Ro5-4864 inhibited prolactin stimulated mitogenesis. Clonazepam, a ligand for the central benzodiazepine receptor had no effect on mitogenesis. Interaction studies were undertaken to determine if Ro5-4864 and PK 11195 act on the same site. The ability of each ligand to enhance the mitogenic action of prolactin was blocked by a 10⁻⁶ M concentration of the other ligand. Finally, simultaneous addition of 10⁻⁹ M of the ligands resulted in no additive effect over each ligand alone. These data show that peripheral benzodiazepine ligands modulate prolactin-stimulated mitogenesis and suggests they interact at the same binding site.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectBenzodiazepines.en_US
dc.subjectCell division.en_US
dc.subjectCell culture.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplinePharmacology and Toxicologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorLaird, Hugh E.en_US
dc.identifier.proquest1339207en_US
dc.identifier.oclc24359506en_US
dc.identifier.bibrecord.b17855640en_US
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