Reaction kinetics of protein hydrolysis, amino acid decomposition, and isoleucine epimerization in eggshell of the African Ostrich, Struthio camelus

Persistent Link:
http://hdl.handle.net/10150/277199
Title:
Reaction kinetics of protein hydrolysis, amino acid decomposition, and isoleucine epimerization in eggshell of the African Ostrich, Struthio camelus
Author:
Ernst, Richard David, 1964-
Issue Date:
1989
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Eggshell of the African Ostrich, Struthio camelus, frequently occurs in African archaeological sites, some of which are beyond the limit of radiocarbon dating. In order to date those sites beyond the range of ¹⁴C dating using amino acid geochemistry, an understanding of the reaction kinetics governing amino acid chemistry must be achieved. The integrity of the eggshell matrix provides an excellent medium to investigate the kinetics of protein hydrolysis, amino acid decomposition, and isoleucine epimerization. Mathematical equations are derived from high temperature simulations of time and well-dated sites at ambient temperatures to determine rate constants for the reactions to a D/L ratio of 1.0. The reactions studied are Free and Total isoleucine epimerization, "classical" and "extended" hydrolysis, and the change in concentrations of free, bound, and total amino acids. Arrhenius plots aided in deriving the equations for each method which are then applied to predict kinetic parameters. Temperatures can be predicted within a 2°C range and time within 15% of its actual value if the appropriate method is used.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Archaeological dating.; Amino acids.; Ostriches -- Eggs.; Racemization.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Geosciences
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleReaction kinetics of protein hydrolysis, amino acid decomposition, and isoleucine epimerization in eggshell of the African Ostrich, Struthio camelusen_US
dc.creatorErnst, Richard David, 1964-en_US
dc.contributor.authorErnst, Richard David, 1964-en_US
dc.date.issued1989en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractEggshell of the African Ostrich, Struthio camelus, frequently occurs in African archaeological sites, some of which are beyond the limit of radiocarbon dating. In order to date those sites beyond the range of ¹⁴C dating using amino acid geochemistry, an understanding of the reaction kinetics governing amino acid chemistry must be achieved. The integrity of the eggshell matrix provides an excellent medium to investigate the kinetics of protein hydrolysis, amino acid decomposition, and isoleucine epimerization. Mathematical equations are derived from high temperature simulations of time and well-dated sites at ambient temperatures to determine rate constants for the reactions to a D/L ratio of 1.0. The reactions studied are Free and Total isoleucine epimerization, "classical" and "extended" hydrolysis, and the change in concentrations of free, bound, and total amino acids. Arrhenius plots aided in deriving the equations for each method which are then applied to predict kinetic parameters. Temperatures can be predicted within a 2°C range and time within 15% of its actual value if the appropriate method is used.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectArchaeological dating.en_US
dc.subjectAmino acids.en_US
dc.subjectOstriches -- Eggs.en_US
dc.subjectRacemization.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineGeosciencesen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.identifier.proquest1339206en_US
dc.identifier.oclc23442278en_US
dc.identifier.bibrecord.b18421167en_US
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