Determination of the toxic/mutagenic potential of toxins associated with ciguatera dinoflagellates

Persistent Link:
http://hdl.handle.net/10150/277164
Title:
Determination of the toxic/mutagenic potential of toxins associated with ciguatera dinoflagellates
Author:
Juranovic, Lillian Regina, 1962-
Issue Date:
1989
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Toxic/mutagenic potentials of Gambierdiscus toxicus (GT) and Prorocentrum lima (PL) methanol extracts (CME) were determined using brine shrimp (Artemia salina), mouse, chicken embryo and Salmonella microsomal assays. PL-CME and GT-CME were toxic to shrimp and mice. Isolation and separation were accomplished using ether/water, hexane/methanol partition and butanol extraction. Toxic fractions were purified using column and thin layer chromatography (TLC). GT-CME showed low levels of mutagenic potential. For GT isolated fractions and PL-CME, no mutagenic effects were observed. Both CMEs showed embryotoxicity, with no teratogenic effects. Ether/methanol and water/butanol fractions showed shrimp toxicity. These fractions were purified by treatment with warm/cold acetone. Acetone insoluble precipitates were obtained. Ether soluble acetone filtrate (ESAF) and butanol soluble acetone precipitate (BSAP) showed shrimp and mouse toxicity. GT-BSAP produced temperature depression in mice. Three toxic isolates were obtained from PL-ESAF, four from GT-ESAF and one from both BSAPs columns. TLC preparative plates showed at least 12 toxic isolates for PL-ESAF, 8 for GT-ESAF and 4 for GT-BSAP.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Food -- Toxicology.; Seafood poisoning.; Poisonous fishes -- Toxicology.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Nutritrion and Food Science
Degree Grantor:
University of Arizona
Advisor:
Park, Douglas L.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleDetermination of the toxic/mutagenic potential of toxins associated with ciguatera dinoflagellatesen_US
dc.creatorJuranovic, Lillian Regina, 1962-en_US
dc.contributor.authorJuranovic, Lillian Regina, 1962-en_US
dc.date.issued1989en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractToxic/mutagenic potentials of Gambierdiscus toxicus (GT) and Prorocentrum lima (PL) methanol extracts (CME) were determined using brine shrimp (Artemia salina), mouse, chicken embryo and Salmonella microsomal assays. PL-CME and GT-CME were toxic to shrimp and mice. Isolation and separation were accomplished using ether/water, hexane/methanol partition and butanol extraction. Toxic fractions were purified using column and thin layer chromatography (TLC). GT-CME showed low levels of mutagenic potential. For GT isolated fractions and PL-CME, no mutagenic effects were observed. Both CMEs showed embryotoxicity, with no teratogenic effects. Ether/methanol and water/butanol fractions showed shrimp toxicity. These fractions were purified by treatment with warm/cold acetone. Acetone insoluble precipitates were obtained. Ether soluble acetone filtrate (ESAF) and butanol soluble acetone precipitate (BSAP) showed shrimp and mouse toxicity. GT-BSAP produced temperature depression in mice. Three toxic isolates were obtained from PL-ESAF, four from GT-ESAF and one from both BSAPs columns. TLC preparative plates showed at least 12 toxic isolates for PL-ESAF, 8 for GT-ESAF and 4 for GT-BSAP.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectFood -- Toxicology.en_US
dc.subjectSeafood poisoning.en_US
dc.subjectPoisonous fishes -- Toxicology.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineNutritrion and Food Scienceen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorPark, Douglas L.en_US
dc.identifier.proquest1339045en_US
dc.identifier.oclc24359035en_US
dc.identifier.bibrecord.b1785555xen_US
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