Fluorescence and elastic scattering from laser dye-filled capillaries

Persistent Link:
http://hdl.handle.net/10150/277121
Title:
Fluorescence and elastic scattering from laser dye-filled capillaries
Author:
Sekerak, Edward Michael, 1959-
Issue Date:
1989
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
We investigated the elastic scattering and fluorescence from laser dye solutions inside 5000, 1100, and 96.5 micron inner-diameter hollow-core capillaries. Incident 4416 A laser illumination of Coumarin 7 dye dissolved in ethanol caused fluorescence from approximately 4600 to 6000 A. This was studied over an angular range from 0° to 360°. A light scattering nephelometer coupled with a spectrometer gave intensity measurements as functions of wavelength (at fixed detection angles) and angle (at fixed wavelengths), while the illumination source, dye-filled capillary, and detector remained stationary. We saw capillary size and detection-angle dependence of the fluorescence and elastic scattering. Results show that angular variations of the elastic scattering and emitted fluorescence can be used to determine an optimum detection angle from the capillary with respect to the incident illumination direction. This work is important in the design and execution of Capillary Zone Electrophoresis (CZE) experiments.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Electrophoresis -- Equipment and supplies -- Design.; Dye lasers.; Fluorescence.; Elastic scattering.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Optical Sciences
Degree Grantor:
University of Arizona
Advisor:
Bickel, William S.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleFluorescence and elastic scattering from laser dye-filled capillariesen_US
dc.creatorSekerak, Edward Michael, 1959-en_US
dc.contributor.authorSekerak, Edward Michael, 1959-en_US
dc.date.issued1989en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractWe investigated the elastic scattering and fluorescence from laser dye solutions inside 5000, 1100, and 96.5 micron inner-diameter hollow-core capillaries. Incident 4416 A laser illumination of Coumarin 7 dye dissolved in ethanol caused fluorescence from approximately 4600 to 6000 A. This was studied over an angular range from 0° to 360°. A light scattering nephelometer coupled with a spectrometer gave intensity measurements as functions of wavelength (at fixed detection angles) and angle (at fixed wavelengths), while the illumination source, dye-filled capillary, and detector remained stationary. We saw capillary size and detection-angle dependence of the fluorescence and elastic scattering. Results show that angular variations of the elastic scattering and emitted fluorescence can be used to determine an optimum detection angle from the capillary with respect to the incident illumination direction. This work is important in the design and execution of Capillary Zone Electrophoresis (CZE) experiments.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectElectrophoresis -- Equipment and supplies -- Design.en_US
dc.subjectDye lasers.en_US
dc.subjectFluorescence.en_US
dc.subjectElastic scattering.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineOptical Sciencesen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorBickel, William S.en_US
dc.identifier.proquest1338084en_US
dc.identifier.oclc23296306en_US
dc.identifier.bibrecord.b1761482xen_US
All Items in UA Campus Repository are protected by copyright, with all rights reserved, unless otherwise indicated.