Effects of diet on amylase content and synthesis in cultured rat acinar cells

Persistent Link:
http://hdl.handle.net/10150/276962
Title:
Effects of diet on amylase content and synthesis in cultured rat acinar cells
Author:
Justice, Jill Diane, 1963-
Issue Date:
1989
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
To study adaptation of pancreatic amylase to diet, an affinity adsorbent, alpha-GHI-AH-Sepharose 4B, was used to determine amylase synthesis in cultured pancreatic acinar cells. This adsorbent exhibited a consistent binding capacity and was specific for amylase. Acinar cells from rats fed high fat (HF) or carbohydrate (HC) diets for 7 d were cultured 1-48 h in serum-free medium. Amylase activity remained significantly higher in HC cells than in HF cells through 24 h in culture, despite its decrease with time in culture. The relative synthesis of amylase (3H-phe amylase/3H-phe total protein x 100) was significantly higher in HC than in HF cells at isolation and remained higher during culture. These results demonstrate that this affinity adsorbent can be used to determine amylase synthesis and suggest that the effect of diet on amylase activity and relative synthesis persists in cultured pancreatic acinar cells.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Amylases -- Secretion -- Regulation.; Pancreatic acinar cells.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Nutritional Sciences
Degree Grantor:
University of Arizona
Advisor:
Brannon, Patsy M.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleEffects of diet on amylase content and synthesis in cultured rat acinar cellsen_US
dc.creatorJustice, Jill Diane, 1963-en_US
dc.contributor.authorJustice, Jill Diane, 1963-en_US
dc.date.issued1989en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractTo study adaptation of pancreatic amylase to diet, an affinity adsorbent, alpha-GHI-AH-Sepharose 4B, was used to determine amylase synthesis in cultured pancreatic acinar cells. This adsorbent exhibited a consistent binding capacity and was specific for amylase. Acinar cells from rats fed high fat (HF) or carbohydrate (HC) diets for 7 d were cultured 1-48 h in serum-free medium. Amylase activity remained significantly higher in HC cells than in HF cells through 24 h in culture, despite its decrease with time in culture. The relative synthesis of amylase (3H-phe amylase/3H-phe total protein x 100) was significantly higher in HC than in HF cells at isolation and remained higher during culture. These results demonstrate that this affinity adsorbent can be used to determine amylase synthesis and suggest that the effect of diet on amylase activity and relative synthesis persists in cultured pancreatic acinar cells.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectAmylases -- Secretion -- Regulation.en_US
dc.subjectPancreatic acinar cells.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineNutritional Sciencesen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorBrannon, Patsy M.en_US
dc.identifier.proquest1336551en_US
dc.identifier.oclc22448700en_US
dc.identifier.bibrecord.b17429560en_US
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