Gene expression in two different genotypes of alfalfa under salt stressed and unstressed conditions

Persistent Link:
http://hdl.handle.net/10150/276936
Title:
Gene expression in two different genotypes of alfalfa under salt stressed and unstressed conditions
Author:
Zheng, Liansheng, 1955-
Issue Date:
1988
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Gene expression in two different genotypes of alfalfa, salt-tolerant and salt-sensitive, was examined by studying differences in protein products coded for by poly(A+) RNA isolated from shoot and root tissue. Plants were grown in hydroponics under unstressed or salt-stressed conditions. Two salinity levels (low salt: 30 mM NaCl and 6 mM CaCl2 and high salt: 133 mM NaCl and 27 mM CaCl2) and one unstressed control were applied. The salt-tolerant genotype showed higher biomass accumulation than the salt-sensitive genotype under both control and salt-stressed conditions. The difference in biomass accumulation between the two genotypes was greatest at the highest salt level. The effect of salt stress on gene expression was studied via in vitro translation of poly (A+) RNA with (35S) -methionine. The labeling pattern was similar in all treatments when analyzed by one dimensional SDS-PAGE. However, a two dimensional analysis (isoelectric focusing followed by SDS-PAGE) showed that salt-stress induced a number of new proteins and repressed several others.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Alfalfa -- Genetics.; Plants -- Effect of salts on.; Plant molecular genetics.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Plant Sciences
Degree Grantor:
University of Arizona
Advisor:
Goldsteinn, Alan H.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleGene expression in two different genotypes of alfalfa under salt stressed and unstressed conditionsen_US
dc.creatorZheng, Liansheng, 1955-en_US
dc.contributor.authorZheng, Liansheng, 1955-en_US
dc.date.issued1988en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractGene expression in two different genotypes of alfalfa, salt-tolerant and salt-sensitive, was examined by studying differences in protein products coded for by poly(A+) RNA isolated from shoot and root tissue. Plants were grown in hydroponics under unstressed or salt-stressed conditions. Two salinity levels (low salt: 30 mM NaCl and 6 mM CaCl2 and high salt: 133 mM NaCl and 27 mM CaCl2) and one unstressed control were applied. The salt-tolerant genotype showed higher biomass accumulation than the salt-sensitive genotype under both control and salt-stressed conditions. The difference in biomass accumulation between the two genotypes was greatest at the highest salt level. The effect of salt stress on gene expression was studied via in vitro translation of poly (A+) RNA with (35S) -methionine. The labeling pattern was similar in all treatments when analyzed by one dimensional SDS-PAGE. However, a two dimensional analysis (isoelectric focusing followed by SDS-PAGE) showed that salt-stress induced a number of new proteins and repressed several others.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectAlfalfa -- Genetics.en_US
dc.subjectPlants -- Effect of salts on.en_US
dc.subjectPlant molecular genetics.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplinePlant Sciencesen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorGoldsteinn, Alan H.en_US
dc.identifier.proquest1335864en_US
dc.identifier.oclc22499390en_US
dc.identifier.bibrecord.b17444421en_US
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