Selection of asparagine substrate analog and sodium-chloride resistant mutants in Arabidopsis thaliana

Persistent Link:
http://hdl.handle.net/10150/276860
Title:
Selection of asparagine substrate analog and sodium-chloride resistant mutants in Arabidopsis thaliana
Author:
Chen, Futai, 1952-
Issue Date:
1988
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The inhibitory effects of NaCl, L- and D-asparagine, and asparagine substrate analogs, beta-aspartyl hydroxamate (AAH) and albizziin, alone or in combination on Columbia Arabidopsis seed germination and seedling survival were characterized under aseptic conditions. Germination on an agar medium supplemented with inorganic nutrients was prevented by 200 mM NaCl, 20 mM L-asparagine, 60 mM D-asparagine, 1.4 mM AAH, or 8 mM albizziin. Established seedlings were generally more tolerant to these chemicals than germinating seeds. Exogenous L- and D-asparagine partly reversed the inhibitory effects of NaCl on seed germination. L-asparagine also partly reversed AAH inhibition of germination. A M2 seed bank was created from the self-pollinated progeny of ethyl methane sulfonate treated seeds. Arabidopsis mutants having increased tolerance to NaCl and AAH, but not albizziin, were successfully selected from this seed bank.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Plants -- Effect of salt on.; Plant mutation.; Arabidopsis thaliana.; Asparaginase.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Plant Sciences
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleSelection of asparagine substrate analog and sodium-chloride resistant mutants in Arabidopsis thalianaen_US
dc.creatorChen, Futai, 1952-en_US
dc.contributor.authorChen, Futai, 1952-en_US
dc.date.issued1988en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe inhibitory effects of NaCl, L- and D-asparagine, and asparagine substrate analogs, beta-aspartyl hydroxamate (AAH) and albizziin, alone or in combination on Columbia Arabidopsis seed germination and seedling survival were characterized under aseptic conditions. Germination on an agar medium supplemented with inorganic nutrients was prevented by 200 mM NaCl, 20 mM L-asparagine, 60 mM D-asparagine, 1.4 mM AAH, or 8 mM albizziin. Established seedlings were generally more tolerant to these chemicals than germinating seeds. Exogenous L- and D-asparagine partly reversed the inhibitory effects of NaCl on seed germination. L-asparagine also partly reversed AAH inhibition of germination. A M2 seed bank was created from the self-pollinated progeny of ethyl methane sulfonate treated seeds. Arabidopsis mutants having increased tolerance to NaCl and AAH, but not albizziin, were successfully selected from this seed bank.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectPlants -- Effect of salt on.en_US
dc.subjectPlant mutation.en_US
dc.subjectArabidopsis thaliana.en_US
dc.subjectAsparaginase.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplinePlant Sciencesen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.identifier.proquest1335672en_US
dc.identifier.oclc22152135en_US
dc.identifier.bibrecord.b17364371en_US
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