Preparation of immunochemical reagents for a non-competitive assay for the detection of haptens

Persistent Link:
http://hdl.handle.net/10150/276829
Title:
Preparation of immunochemical reagents for a non-competitive assay for the detection of haptens
Author:
Terhune, Tammy Lee, 1962-
Issue Date:
1988
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The strategy required for the development of the immunochemical reagents needed for a non-competitive assay for the detection of haptens was investigated. For this work, these reagents were termed "anti-complex" antibodies. To preserve the integrity of such an assay, these "anti-complex" antibodies should not react with either the free anti-hapten antibody or the free hapten and they should not compete with the hapten for binding to the anti-hapten antibody. Anti-hapten hybridomas were developed using standard hybridoma technology. The development of anti-complex hybridomas was investigated using standard hybridoma technology and the novel approach of intrasplenic immunization. Both immunization protocols addressed the three questions concerning the strategy for developing such hybridomas, namely the type of immunization (snygeneic or allogeneic), the physico-chemical nature of the hapten in the complex (free or conjugated) and the physico-chemical nature of the anti-hapten antibody in the complex (intact or fragmented). (Abstract shortened with permission of author.)
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Haptens -- Analysis.; Immunoassay.; Chemical tests and reagents.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Chemistry
Degree Grantor:
University of Arizona
Advisor:
Wilson, George S.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titlePreparation of immunochemical reagents for a non-competitive assay for the detection of haptensen_US
dc.creatorTerhune, Tammy Lee, 1962-en_US
dc.contributor.authorTerhune, Tammy Lee, 1962-en_US
dc.date.issued1988en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe strategy required for the development of the immunochemical reagents needed for a non-competitive assay for the detection of haptens was investigated. For this work, these reagents were termed "anti-complex" antibodies. To preserve the integrity of such an assay, these "anti-complex" antibodies should not react with either the free anti-hapten antibody or the free hapten and they should not compete with the hapten for binding to the anti-hapten antibody. Anti-hapten hybridomas were developed using standard hybridoma technology. The development of anti-complex hybridomas was investigated using standard hybridoma technology and the novel approach of intrasplenic immunization. Both immunization protocols addressed the three questions concerning the strategy for developing such hybridomas, namely the type of immunization (snygeneic or allogeneic), the physico-chemical nature of the hapten in the complex (free or conjugated) and the physico-chemical nature of the anti-hapten antibody in the complex (intact or fragmented). (Abstract shortened with permission of author.)en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectHaptens -- Analysis.en_US
dc.subjectImmunoassay.en_US
dc.subjectChemical tests and reagents.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineChemistryen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorWilson, George S.en_US
dc.identifier.proquest1335393en_US
dc.identifier.oclc21893924en_US
dc.identifier.bibrecord.b17321189en_US
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