Control of fibroblast contamination in primary rat skeletal muscle cell cultures: Effects of an epidermal growth factor linked cytotoxin

Persistent Link:
http://hdl.handle.net/10150/276812
Title:
Control of fibroblast contamination in primary rat skeletal muscle cell cultures: Effects of an epidermal growth factor linked cytotoxin
Author:
Pierce, Paul Randall, 1951-
Issue Date:
1988
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The in vitro study of muscle cell growth is hampered by the presence of non-muscle cells, particularly fibroblasts. The heterobifunctional cross-linking agent, N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) has been used to create a novel "toxic growth factor" to address the problem. Epidermal growth factor (EGF), which stimulates fibroblast but not satellite cell proliferation, was conjugated via SPDP to a potent ribosome inhibitor, pokeweed antiviral protein (PAP). By preferentially binding to fibroblasts, it was hoped that EGF-PAP could cytotoxically eliminate fibroblasts from primary cultures of rat skeletal muscle satellite cells. While EGF-PAP did prove to be a fibroblast cytotoxin, it could not completely eliminate them from cell cultures. Low dose-time exposures improved the ratio of multinucleated cells to mononucleated cells (percent fusion) by up to 66% over controls, but increased concentrations, or durations of EGF-PAP treatment, proved detrimental to satellite cell growth and/or differentiation.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Muscle cells -- Growth.; Growth factors.; Fibroblasts.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Animal Physiology
Degree Grantor:
University of Arizona
Advisor:
Allen, Ronald E.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleControl of fibroblast contamination in primary rat skeletal muscle cell cultures: Effects of an epidermal growth factor linked cytotoxinen_US
dc.creatorPierce, Paul Randall, 1951-en_US
dc.contributor.authorPierce, Paul Randall, 1951-en_US
dc.date.issued1988en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe in vitro study of muscle cell growth is hampered by the presence of non-muscle cells, particularly fibroblasts. The heterobifunctional cross-linking agent, N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) has been used to create a novel "toxic growth factor" to address the problem. Epidermal growth factor (EGF), which stimulates fibroblast but not satellite cell proliferation, was conjugated via SPDP to a potent ribosome inhibitor, pokeweed antiviral protein (PAP). By preferentially binding to fibroblasts, it was hoped that EGF-PAP could cytotoxically eliminate fibroblasts from primary cultures of rat skeletal muscle satellite cells. While EGF-PAP did prove to be a fibroblast cytotoxin, it could not completely eliminate them from cell cultures. Low dose-time exposures improved the ratio of multinucleated cells to mononucleated cells (percent fusion) by up to 66% over controls, but increased concentrations, or durations of EGF-PAP treatment, proved detrimental to satellite cell growth and/or differentiation.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectMuscle cells -- Growth.en_US
dc.subjectGrowth factors.en_US
dc.subjectFibroblasts.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineAnimal Physiologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorAllen, Ronald E.en_US
dc.identifier.proquest1335059en_US
dc.identifier.oclc22350670en_US
dc.identifier.bibrecord.b17413217en_US
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