Persistent Link:
http://hdl.handle.net/10150/276811
Title:
Acclimation of mixed cultures for phenol biodegradation
Author:
Phillips, David Gray, 1949-
Issue Date:
1988
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Experiments were conducted to examine the cause of lag-phase growth during phenol degradation by mixed microbial cultures that had been acclimated to one of four substrates. Four aerated Imhoff cones were inoculated with wastewater sludge and fed one of four substrates: acetate, egg albumin, vegetable oil, or phenol. Inocula from these cones were injected into batch reactors containing phenol. Time-dependent growth was measured by two methods: most probable number (MPN) and epifluorescence microscopy (EM). The MPN technique was used to distinguish two cell concentrations: total cells and a phenol-degrading community within the total; EM was also used to count total cells. The results indicated that a lag in phenol utilization for all cultures, except the phenol-acclimated cultures, was a result of growth of a phenol-degrading subpopulation, and not due to enzyme induction of the existing population. Similar experiments were conducted using 2,4-dichlorophenol (2,4-DCP), which resulted in no growth and no degradation of 2,4-DCP.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Phenol -- Metabolic detoxification.; Phenol -- Biodegradation.; Xenobiotics.; Microbial metabolism.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Civil Engineering and Engineering Mechanics
Degree Grantor:
University of Arizona
Advisor:
Logan, Bruce E.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleAcclimation of mixed cultures for phenol biodegradationen_US
dc.creatorPhillips, David Gray, 1949-en_US
dc.contributor.authorPhillips, David Gray, 1949-en_US
dc.date.issued1988en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractExperiments were conducted to examine the cause of lag-phase growth during phenol degradation by mixed microbial cultures that had been acclimated to one of four substrates. Four aerated Imhoff cones were inoculated with wastewater sludge and fed one of four substrates: acetate, egg albumin, vegetable oil, or phenol. Inocula from these cones were injected into batch reactors containing phenol. Time-dependent growth was measured by two methods: most probable number (MPN) and epifluorescence microscopy (EM). The MPN technique was used to distinguish two cell concentrations: total cells and a phenol-degrading community within the total; EM was also used to count total cells. The results indicated that a lag in phenol utilization for all cultures, except the phenol-acclimated cultures, was a result of growth of a phenol-degrading subpopulation, and not due to enzyme induction of the existing population. Similar experiments were conducted using 2,4-dichlorophenol (2,4-DCP), which resulted in no growth and no degradation of 2,4-DCP.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectPhenol -- Metabolic detoxification.en_US
dc.subjectPhenol -- Biodegradation.en_US
dc.subjectXenobiotics.en_US
dc.subjectMicrobial metabolism.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineCivil Engineering and Engineering Mechanicsen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorLogan, Bruce E.en_US
dc.identifier.proquest1335058en_US
dc.identifier.oclc21540344en_US
dc.identifier.bibrecord.b17272038en_US
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