Subcellular distribution and binding characterization of the peripheral benzodiazepine receptor in the rat liver

Persistent Link:
http://hdl.handle.net/10150/276794
Title:
Subcellular distribution and binding characterization of the peripheral benzodiazepine receptor in the rat liver
Author:
Duerson, Kevin Collin, 1958-
Issue Date:
1988
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The peripheral benzodiazepine receptor (PBZ) population in the rat liver has been kinetically and pharmacologically characterized. These data revealed a receptor with a dissociation constant (Kd) of 5.67 nM and a receptor density of 758 fmoles/ mg protein in a crude membrane preparation. Subcellular distribution studies demonstrated that PBZs were detected in highly purified nuclear, mitochondrial and microsomal fractions. Porphyrins were shown to interact at the PBZ with protoporphyrin IX and hemin having the highest affinity. Preliminary studies on the subnuclear distribution of PBZs are reported. In summary, the study has (1) characterized liver PBZs, (2) shown the subcellular distribution of these receptors and (3) pharmacologically characterized nuclear, mitochondrial and microsomal PBZs in the liver.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Benzodiazepines -- Receptors.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Toxicology
Degree Grantor:
University of Arizona
Advisor:
Laird, Hugh E.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleSubcellular distribution and binding characterization of the peripheral benzodiazepine receptor in the rat liveren_US
dc.creatorDuerson, Kevin Collin, 1958-en_US
dc.contributor.authorDuerson, Kevin Collin, 1958-en_US
dc.date.issued1988en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe peripheral benzodiazepine receptor (PBZ) population in the rat liver has been kinetically and pharmacologically characterized. These data revealed a receptor with a dissociation constant (Kd) of 5.67 nM and a receptor density of 758 fmoles/ mg protein in a crude membrane preparation. Subcellular distribution studies demonstrated that PBZs were detected in highly purified nuclear, mitochondrial and microsomal fractions. Porphyrins were shown to interact at the PBZ with protoporphyrin IX and hemin having the highest affinity. Preliminary studies on the subnuclear distribution of PBZs are reported. In summary, the study has (1) characterized liver PBZs, (2) shown the subcellular distribution of these receptors and (3) pharmacologically characterized nuclear, mitochondrial and microsomal PBZs in the liver.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectBenzodiazepines -- Receptors.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineToxicologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorLaird, Hugh E.en_US
dc.identifier.proquest1334349en_US
dc.identifier.oclc22204423en_US
dc.identifier.bibrecord.b17375277en_US
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