Production and in vitro characterization of antibody against acetaldehyde rabbit serum albumin conjugates

Persistent Link:
http://hdl.handle.net/10150/276603
Title:
Production and in vitro characterization of antibody against acetaldehyde rabbit serum albumin conjugates
Author:
Lung, Chien-Cheng, 1960-
Issue Date:
1987
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Acetaldehyde, the first metabolite of ethanol, has been implicated in the pathogenesis of alcoholic liver disease. In order to investigate a possible immunologic mechanism whereby acetaldehyde might exert its toxic effect acetaldehyde protein conjuates were prepared and characterized. This study demonstrates that acetaldehyde conjugated albumin can be an immunogen, can form a more negatively charged, more acidic, heterogeneous conjugate than albumin and can elicitate a specific rabbit antibody. ELISA can be used to assay antibodies produced in response to acetaldehyde albumin conjugates suggesting that chronic alcohol ingestion can lead the generation of antibodies against acetaldehyde conjugated human serum albumin. The significance of this study is that it possibly can provide a method to investigate the mechanism responsible for the sequelae of alcoholism.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Alcohol -- Physiological effect.; Immunotoxicology.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Microbiology and Immunology
Degree Grantor:
University of Arizona
Advisor:
Pinnas, Jacob L.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleProduction and in vitro characterization of antibody against acetaldehyde rabbit serum albumin conjugatesen_US
dc.creatorLung, Chien-Cheng, 1960-en_US
dc.contributor.authorLung, Chien-Cheng, 1960-en_US
dc.date.issued1987en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractAcetaldehyde, the first metabolite of ethanol, has been implicated in the pathogenesis of alcoholic liver disease. In order to investigate a possible immunologic mechanism whereby acetaldehyde might exert its toxic effect acetaldehyde protein conjuates were prepared and characterized. This study demonstrates that acetaldehyde conjugated albumin can be an immunogen, can form a more negatively charged, more acidic, heterogeneous conjugate than albumin and can elicitate a specific rabbit antibody. ELISA can be used to assay antibodies produced in response to acetaldehyde albumin conjugates suggesting that chronic alcohol ingestion can lead the generation of antibodies against acetaldehyde conjugated human serum albumin. The significance of this study is that it possibly can provide a method to investigate the mechanism responsible for the sequelae of alcoholism.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectAlcohol -- Physiological effect.en_US
dc.subjectImmunotoxicology.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineMicrobiology and Immunologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorPinnas, Jacob L.en_US
dc.identifier.proquest1332468en_US
dc.identifier.oclc19232497en_US
dc.identifier.bibrecord.b16733174en_US
All Items in UA Campus Repository are protected by copyright, with all rights reserved, unless otherwise indicated.