LIPOXYGENASE ACTIVITY ASSOCIATED WITH CYANIDE-INSENSITIVE OXYGEN UPTAKE IN MITOCHONDRIAL FRACTIONS FROM SEEDLINGS OF GLYCINE MAX L

Persistent Link:
http://hdl.handle.net/10150/276512
Title:
LIPOXYGENASE ACTIVITY ASSOCIATED WITH CYANIDE-INSENSITIVE OXYGEN UPTAKE IN MITOCHONDRIAL FRACTIONS FROM SEEDLINGS OF GLYCINE MAX L
Author:
Scherban, Donna Michele, 1954-
Issue Date:
1987
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Soybean seeds are known to contain high levels of lipoxygenase activity, especially during early stages of germination. Crude mitochondrial fractions from germinating soybeans also have been shown to exhibit high rates of cyanide-insensitive oxygen uptake. These results show the effects of successive discontinuous PercollR density gradients on mitochondrial fractions from 2 day old soybean seeds as judged by polarographic studies and Ouchterlony double diffusion. Axis mitochondria exhibited totally cyanide-sensitive oxygen uptake after two gradients and cotyledon mitochondria exhibited from none to 11% cyanide-insensitive oxygen uptake after three gradients. Mitochondrial fractions which were assayed for lipoxygenase with double diffusion exhibited positive results with fractions that showed cyanide-insensitive oxygen uptake and negative results with cyanide-sensitive mitochondria. These results suggest that lipoxygenase can loosely associate with the mitochondrial membrane and that gradient centrifugation can purify mitochondria free of both lipoxygenase and cyanide-insensitive oxygen uptake.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Lipoxygenases.; Soybean -- Seedlings.; Plant mitochondria.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Plant Sciences
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleLIPOXYGENASE ACTIVITY ASSOCIATED WITH CYANIDE-INSENSITIVE OXYGEN UPTAKE IN MITOCHONDRIAL FRACTIONS FROM SEEDLINGS OF GLYCINE MAX Len_US
dc.creatorScherban, Donna Michele, 1954-en_US
dc.contributor.authorScherban, Donna Michele, 1954-en_US
dc.date.issued1987en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractSoybean seeds are known to contain high levels of lipoxygenase activity, especially during early stages of germination. Crude mitochondrial fractions from germinating soybeans also have been shown to exhibit high rates of cyanide-insensitive oxygen uptake. These results show the effects of successive discontinuous PercollR density gradients on mitochondrial fractions from 2 day old soybean seeds as judged by polarographic studies and Ouchterlony double diffusion. Axis mitochondria exhibited totally cyanide-sensitive oxygen uptake after two gradients and cotyledon mitochondria exhibited from none to 11% cyanide-insensitive oxygen uptake after three gradients. Mitochondrial fractions which were assayed for lipoxygenase with double diffusion exhibited positive results with fractions that showed cyanide-insensitive oxygen uptake and negative results with cyanide-sensitive mitochondria. These results suggest that lipoxygenase can loosely associate with the mitochondrial membrane and that gradient centrifugation can purify mitochondria free of both lipoxygenase and cyanide-insensitive oxygen uptake.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectLipoxygenases.en_US
dc.subjectSoybean -- Seedlings.en_US
dc.subjectPlant mitochondria.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplinePlant Sciencesen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.identifier.proquest1331470en_US
dc.identifier.oclc18158095en_US
dc.identifier.bibrecord.b16483406en_US
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