RESPONSE OF THE TEPARY BEAN PHASEOLUS ACUTIFOLIUS A. GREY, TO TISSUE CULTURE SYSTEMS

Persistent Link:
http://hdl.handle.net/10150/276474
Title:
RESPONSE OF THE TEPARY BEAN PHASEOLUS ACUTIFOLIUS A. GREY, TO TISSUE CULTURE SYSTEMS
Author:
Lormand, Katherine Bradbury, 1961-
Issue Date:
1987
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The responses of the tepary bean (Phaseolus acutifolius) to in vitro tissue culture systems were documented. Tests were conducted to identify the optimal auxin and cytokinin combinations required for optimal callus growth. Regeneration experiments were conducted to: (1) determine the effect of explant source and age on regeneration, (2) effect of callus age on regeneration, (3) the cultivation status of the explant source, and (4) the effects of nutritional additives on somatic embryogenesis. The callus was easily induced and maintained in all hormonal medias except those containing IAA and 6BA. The results for regeneration were most promising from cultures derived from immature cotyledon tissue. Ammonium Chloride, glutamine and Absisic acid appeared to have little affect on embryogenesis, however the addition of kinetin enabled the embryos to develop to the torpedo stage. Callus age and cultivative status of explant source had no effects on plantlet regeneration.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Tepary bean -- Micropropagation.; Plant tissue culture.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Plant Sciences
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleRESPONSE OF THE TEPARY BEAN PHASEOLUS ACUTIFOLIUS A. GREY, TO TISSUE CULTURE SYSTEMSen_US
dc.creatorLormand, Katherine Bradbury, 1961-en_US
dc.contributor.authorLormand, Katherine Bradbury, 1961-en_US
dc.date.issued1987en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe responses of the tepary bean (Phaseolus acutifolius) to in vitro tissue culture systems were documented. Tests were conducted to identify the optimal auxin and cytokinin combinations required for optimal callus growth. Regeneration experiments were conducted to: (1) determine the effect of explant source and age on regeneration, (2) effect of callus age on regeneration, (3) the cultivation status of the explant source, and (4) the effects of nutritional additives on somatic embryogenesis. The callus was easily induced and maintained in all hormonal medias except those containing IAA and 6BA. The results for regeneration were most promising from cultures derived from immature cotyledon tissue. Ammonium Chloride, glutamine and Absisic acid appeared to have little affect on embryogenesis, however the addition of kinetin enabled the embryos to develop to the torpedo stage. Callus age and cultivative status of explant source had no effects on plantlet regeneration.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectTepary bean -- Micropropagation.en_US
dc.subjectPlant tissue culture.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplinePlant Sciencesen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.identifier.proquest1331415en_US
dc.identifier.oclc18826120en_US
dc.identifier.bibrecord.b16648948en_US
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