EFFECT OF RECOMBINANT INTERLEUKIN 2 ON DAUDI CELL KILLING IN NEWBORNS

Persistent Link:
http://hdl.handle.net/10150/276458
Title:
EFFECT OF RECOMBINANT INTERLEUKIN 2 ON DAUDI CELL KILLING IN NEWBORNS
Author:
Freitag, Lori Linn, 1959-
Issue Date:
1987
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Experiments were done to determine the effect of recombinant interleukin 2 (rIL-2) on mononuclear cells (MC) of newborns and adults. MC were tested for (1) ability to lyse Daudi cells in a 51Cr release assay, (2) cell surface markers using monoclonal antibodies and flow cytometer analysis, and (3) cell types as determined by differential cell counts. Without rIL-2 adults show greater cytotoxicity than newborns in vitro. Incubation with rIL-2 dramatically increased the cytotoxicity expressed with cord blood and adult MC showing equivalent responses. Differences in cell surface markers between newborns and adults prior to rIL-2 exposure were in agreement with those previously published. This study did not demonstrate changes in phenotypes after exposure to rIL-2. Slight changes in differential cell counts occurred after increased incubation periods and rIL-2 exposure.
Type:
text; Thesis-Reproduction (electronic)
Keywords:
Interleukins.; Immune response -- Regulation.; Lymphocytes.; Cell-mediated cytotoxicity.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Microbiology and Immunology
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_US
dc.titleEFFECT OF RECOMBINANT INTERLEUKIN 2 ON DAUDI CELL KILLING IN NEWBORNSen_US
dc.creatorFreitag, Lori Linn, 1959-en_US
dc.contributor.authorFreitag, Lori Linn, 1959-en_US
dc.date.issued1987en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractExperiments were done to determine the effect of recombinant interleukin 2 (rIL-2) on mononuclear cells (MC) of newborns and adults. MC were tested for (1) ability to lyse Daudi cells in a 51Cr release assay, (2) cell surface markers using monoclonal antibodies and flow cytometer analysis, and (3) cell types as determined by differential cell counts. Without rIL-2 adults show greater cytotoxicity than newborns in vitro. Incubation with rIL-2 dramatically increased the cytotoxicity expressed with cord blood and adult MC showing equivalent responses. Differences in cell surface markers between newborns and adults prior to rIL-2 exposure were in agreement with those previously published. This study did not demonstrate changes in phenotypes after exposure to rIL-2. Slight changes in differential cell counts occurred after increased incubation periods and rIL-2 exposure.en_US
dc.typetexten_US
dc.typeThesis-Reproduction (electronic)en_US
dc.subjectInterleukins.en_US
dc.subjectImmune response -- Regulation.en_US
dc.subjectLymphocytes.en_US
dc.subjectCell-mediated cytotoxicity.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineMicrobiology and Immunologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.identifier.proquest1331400en_US
dc.identifier.oclc17666735en_US
dc.identifier.bibrecord.b16358260en_US
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