Persistent Link:
http://hdl.handle.net/10150/245082
Title:
Role of NADPH Oxidase in Oxidative Stress and Aging
Author:
Tsirklin, Liana
Issue Date:
May-2012
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
As people age, their preferential adipose tissue stores tend to shift from subcutaneous to visceral, which could lead to diabetes, since visceral adipose tissue is insulin resistant. One of the proposed mechanisms for this shift is due to elevated levels of oxidative stress in adipose tissue cells due to aging, as a result of increased levels of NADPH oxidase (NOX). The goal of these experiments is to determine the existence and significance of this potential correlation. Visceral, subcutaneous, and stromal vascular adipose tissue samples from 18 week and 20 month old mice were probed for p47phox levels, a crucial component of NOX, and the levels of oxidative stress were also determined through a dinitrophenolhydrazine reaction in the OxyBlot analysis. In order to achieve these goals, electrophoresis gels were run, the gels were transferred through standard Western blot procedures, then probed with primary and secondary antibodies, and finally scanned and analyzed to determine differences in signal. As of yet, there have not been any statistically significant differences in oxidative stress levels determined in young and old mice, but further experiments will be conducted in order to validate these findings.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Biophysics
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleRole of NADPH Oxidase in Oxidative Stress and Agingen_US
dc.creatorTsirklin, Lianaen_US
dc.contributor.authorTsirklin, Lianaen_US
dc.date.issued2012-05-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractAs people age, their preferential adipose tissue stores tend to shift from subcutaneous to visceral, which could lead to diabetes, since visceral adipose tissue is insulin resistant. One of the proposed mechanisms for this shift is due to elevated levels of oxidative stress in adipose tissue cells due to aging, as a result of increased levels of NADPH oxidase (NOX). The goal of these experiments is to determine the existence and significance of this potential correlation. Visceral, subcutaneous, and stromal vascular adipose tissue samples from 18 week and 20 month old mice were probed for p47phox levels, a crucial component of NOX, and the levels of oxidative stress were also determined through a dinitrophenolhydrazine reaction in the OxyBlot analysis. In order to achieve these goals, electrophoresis gels were run, the gels were transferred through standard Western blot procedures, then probed with primary and secondary antibodies, and finally scanned and analyzed to determine differences in signal. As of yet, there have not been any statistically significant differences in oxidative stress levels determined in young and old mice, but further experiments will be conducted in order to validate these findings.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineBiophysicsen_US
thesis.degree.grantorUniversity of Arizonaen_US
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