Quantitation of Cryptosporidium hominis Oocyst Shedding in a Rodent Model of Human Cryptosporidiosis by Real Time Polymerase Chain Reaction

Persistent Link:
http://hdl.handle.net/10150/244822
Title:
Quantitation of Cryptosporidium hominis Oocyst Shedding in a Rodent Model of Human Cryptosporidiosis by Real Time Polymerase Chain Reaction
Author:
Wyman, Taylor Dawn
Issue Date:
May-2012
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
A real time or quantitative polymerase chain reaction (rtPCR or qPCR) was developed to quantitate Cryptosporidium hominis oocysts shed in the feces of experimentally infected gerbils serving as a model for human cryptosporidiosis. Primers specific for the Cryptosporidium oocyst wall protein (COWP), a gene with 99% conservation between C. hominis and C. parvum, were used to detect DNA extracted from both C. hominis and C. parvum. The concentration of oocysts in the experimental fecal samples was calculated by sequence detection software, comparing these samples to standard curves generated from DNA extracted from purified C. parvum or C. hominis oocysts. The data show a trend of similar quantities when comparing the number of oocysts per ml found using qPCR to the number counted microscopically by immunofluorescence assay. With further optimization, this protocol should be useful for quantitating oocysts in experimental fecal samples from other animal models of cryptosporidiosis resulting from C. hominis and C. parvum infection.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Veterinary Science
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleQuantitation of Cryptosporidium hominis Oocyst Shedding in a Rodent Model of Human Cryptosporidiosis by Real Time Polymerase Chain Reactionen_US
dc.creatorWyman, Taylor Dawnen_US
dc.contributor.authorWyman, Taylor Dawnen_US
dc.date.issued2012-05-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractA real time or quantitative polymerase chain reaction (rtPCR or qPCR) was developed to quantitate Cryptosporidium hominis oocysts shed in the feces of experimentally infected gerbils serving as a model for human cryptosporidiosis. Primers specific for the Cryptosporidium oocyst wall protein (COWP), a gene with 99% conservation between C. hominis and C. parvum, were used to detect DNA extracted from both C. hominis and C. parvum. The concentration of oocysts in the experimental fecal samples was calculated by sequence detection software, comparing these samples to standard curves generated from DNA extracted from purified C. parvum or C. hominis oocysts. The data show a trend of similar quantities when comparing the number of oocysts per ml found using qPCR to the number counted microscopically by immunofluorescence assay. With further optimization, this protocol should be useful for quantitating oocysts in experimental fecal samples from other animal models of cryptosporidiosis resulting from C. hominis and C. parvum infection.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineVeterinary Scienceen_US
thesis.degree.grantorUniversity of Arizonaen_US
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