Characterization of a Gene Targeted by Mitochondrial Retrograde Regulaiton in Arabidopsis Thaliana

Persistent Link:
http://hdl.handle.net/10150/244779
Title:
Characterization of a Gene Targeted by Mitochondrial Retrograde Regulaiton in Arabidopsis Thaliana
Author:
Sepulveda, Jennifer Ashley
Issue Date:
May-2012
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
When under environmental stresses such as pathogen attack, plant mitochondria initiate a signaling pathway, called mitochondrial retrograde regulation (MRR), to alter nuclear gene expression in order to help the plant survive the stress (4). The Rhoads Lab identified an Arabidopsis thaliana (Arabidopsis) gene (AtSg40690, which may encode an A TP synthase assembly protein) that is strongly increased in expression by MRR and during plant stresses. This project was focused on the development of constitutive expressor (CE) lines of AtSg40690. Only one CE line was generated due to low transformation rates, which might indicate lethality. It is possible that transformants might induce a change in another protein, causing variegation in plants. Using classical molecular biology and microbial genetics techniques, the research group plans to verify the expression patterns in the CE lines and knock out (KO) lines of AtSg40690 and test KO lines and other A TP synthase assembly protein (AAP) gene over-expressor lines to determine if these genes play a role in MRR and in stress responses in plants.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Molecular and Cellular Biology
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleCharacterization of a Gene Targeted by Mitochondrial Retrograde Regulaiton in Arabidopsis Thalianaen_US
dc.creatorSepulveda, Jennifer Ashleyen_US
dc.contributor.authorSepulveda, Jennifer Ashleyen_US
dc.date.issued2012-05-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractWhen under environmental stresses such as pathogen attack, plant mitochondria initiate a signaling pathway, called mitochondrial retrograde regulation (MRR), to alter nuclear gene expression in order to help the plant survive the stress (4). The Rhoads Lab identified an Arabidopsis thaliana (Arabidopsis) gene (AtSg40690, which may encode an A TP synthase assembly protein) that is strongly increased in expression by MRR and during plant stresses. This project was focused on the development of constitutive expressor (CE) lines of AtSg40690. Only one CE line was generated due to low transformation rates, which might indicate lethality. It is possible that transformants might induce a change in another protein, causing variegation in plants. Using classical molecular biology and microbial genetics techniques, the research group plans to verify the expression patterns in the CE lines and knock out (KO) lines of AtSg40690 and test KO lines and other A TP synthase assembly protein (AAP) gene over-expressor lines to determine if these genes play a role in MRR and in stress responses in plants.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineMolecular and Cellular Biologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
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