Roles of the RNA-Binding Protein TDP-43 in Sleep and Locomotor Activity in Drosophila

Persistent Link:
http://hdl.handle.net/10150/244472
Title:
Roles of the RNA-Binding Protein TDP-43 in Sleep and Locomotor Activity in Drosophila
Author:
McCallum, Abigail, Priestley
Issue Date:
May-2012
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Amyotrophic lateral sclerosis (ALS) is a lethal, progressive neurodegenerative disease that leads to death of motors neurons, loss of voluntary muscles, respiratory failure, progressive muscle paralysis and death within 3-5 years. In the recent past, the RNA binding protein TDP-43 has emerged as a major player in the pathology of this fatal disease due to its linkage to the majority of ALS cases known to date. This discovery has led to an avalanche of research investigating the role of TDP-43 in ALS. Here, we use the Drosophila Activity Monitoring (DAM) system to investigate sleep and locomotor behavior in adult flies expressing wild type or mutant variants of TDP-43 in motor neurons or glia. We show that activity is significantly decreased in all variants compared to the control, and that average total sleep is increased in wild-type TDP-43 flies but decreased in mutant flies. Furthermore mutant and wild-type flies exhibit an increased number of sleep bouts and a decreased length of sleep bout during the day and night compared to the control. These newly discovered sleep phenotypes in the Drosophila model resemble restlessness and sleeplessness reported in ALS patients and open up new avenues of study for the role of TDP-43 in motor neuron disease and neurodegeneration.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Molecular and Cellular Biology
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleRoles of the RNA-Binding Protein TDP-43 in Sleep and Locomotor Activity in Drosophilaen_US
dc.creatorMcCallum, Abigail, Priestleyen_US
dc.contributor.authorMcCallum, Abigail, Priestleyen_US
dc.date.issued2012-05-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractAmyotrophic lateral sclerosis (ALS) is a lethal, progressive neurodegenerative disease that leads to death of motors neurons, loss of voluntary muscles, respiratory failure, progressive muscle paralysis and death within 3-5 years. In the recent past, the RNA binding protein TDP-43 has emerged as a major player in the pathology of this fatal disease due to its linkage to the majority of ALS cases known to date. This discovery has led to an avalanche of research investigating the role of TDP-43 in ALS. Here, we use the Drosophila Activity Monitoring (DAM) system to investigate sleep and locomotor behavior in adult flies expressing wild type or mutant variants of TDP-43 in motor neurons or glia. We show that activity is significantly decreased in all variants compared to the control, and that average total sleep is increased in wild-type TDP-43 flies but decreased in mutant flies. Furthermore mutant and wild-type flies exhibit an increased number of sleep bouts and a decreased length of sleep bout during the day and night compared to the control. These newly discovered sleep phenotypes in the Drosophila model resemble restlessness and sleeplessness reported in ALS patients and open up new avenues of study for the role of TDP-43 in motor neuron disease and neurodegeneration.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineMolecular and Cellular Biologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
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