Effects of Proinflammtory Cytokines on the Expression of an Apical Bile Acid Transporter Involved in Nectrotizing Entercolitis

Persistent Link:
http://hdl.handle.net/10150/244454
Title:
Effects of Proinflammtory Cytokines on the Expression of an Apical Bile Acid Transporter Involved in Nectrotizing Entercolitis
Author:
Martin, Nina A.
Issue Date:
May-2012
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Necrotizing enterocolitis (NEC) is a severe pediatric gastrointestinal disease that occurs almost exclusively in premature infants, with death rate estimated at 25% . The disease is characterized by inflammation and necrosis of the lower small intestine; however, the pathogenesis of NEC is unknown, making it an important topic for study. Previous studies have shown that during development of NEC, bile acids (compounds secreted by the liver and necessary for nutrient absorption), are increased, as well as the apical membrane transport protein, ASBT, responsible for bile acid reuptake. It is bile acid accumulation inside the cell that is believed to initiate the cellular damage observed in this disease. It has been shown that, in systems where ASBT levels are normally expressed, proinflammatory cytokines and inflammation decrease ASBT promoter activity, but it still remains to be investigated what effect inflammatory cytokines have on ASBT expression under conditions found in NEC. This project will investigate the effects, if any, that proinflammatory cytokines IL‐18 and TNF‐α have on the expression of ASBT in a neonatal rat intestinal cell line that over‐expresses ASBT. The model used will be a tetracycline‐inducible recombinant IEC‐6 cell line, to replicate elevated ASBT expression found in NEC.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Biochemistry and Molecular Biophysics
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleEffects of Proinflammtory Cytokines on the Expression of an Apical Bile Acid Transporter Involved in Nectrotizing Entercolitisen_US
dc.creatorMartin, Nina A.en_US
dc.contributor.authorMartin, Nina A.en_US
dc.date.issued2012-05-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractNecrotizing enterocolitis (NEC) is a severe pediatric gastrointestinal disease that occurs almost exclusively in premature infants, with death rate estimated at 25% . The disease is characterized by inflammation and necrosis of the lower small intestine; however, the pathogenesis of NEC is unknown, making it an important topic for study. Previous studies have shown that during development of NEC, bile acids (compounds secreted by the liver and necessary for nutrient absorption), are increased, as well as the apical membrane transport protein, ASBT, responsible for bile acid reuptake. It is bile acid accumulation inside the cell that is believed to initiate the cellular damage observed in this disease. It has been shown that, in systems where ASBT levels are normally expressed, proinflammatory cytokines and inflammation decrease ASBT promoter activity, but it still remains to be investigated what effect inflammatory cytokines have on ASBT expression under conditions found in NEC. This project will investigate the effects, if any, that proinflammatory cytokines IL‐18 and TNF‐α have on the expression of ASBT in a neonatal rat intestinal cell line that over‐expresses ASBT. The model used will be a tetracycline‐inducible recombinant IEC‐6 cell line, to replicate elevated ASBT expression found in NEC.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineBiochemistry and Molecular Biophysicsen_US
thesis.degree.grantorUniversity of Arizonaen_US
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