Analysis of CREs of ETS1/2 Target Genes in the Basal Chordate Ciona Intestinalis

Persistent Link:
http://hdl.handle.net/10150/244408
Title:
Analysis of CREs of ETS1/2 Target Genes in the Basal Chordate Ciona Intestinalis
Author:
Li, Younan
Issue Date:
May-2012
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Fibroblast growth factor (FGF) signaling and downstream activation of the transcription factor ETS1/2 specify the heart cell lineage in the basal chordate Ciona intestinalis. Based on our previous work, 144 candidate FGF/ETS1/2 dependent heart genes have been identified from the Ciona genome. The expression of a large subset of these genes has been verified in the heart precursor lineage using in-situ techniques. Using an bio-informatic approach, we identified candidate enhancers for some of these target genes. We then tested these predicted enhancers through reporter analysis. Our results indicate that the Ets1/2 binding site GGAW and the co-motif ATTA are required for heart lineage enhancer activity. This work can help us understand the conserved, primary role of FGF/Ets in chordate heart lineage specification.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Molecular and Cellular Biology
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleAnalysis of CREs of ETS1/2 Target Genes in the Basal Chordate Ciona Intestinalisen_US
dc.creatorLi, Younanen_US
dc.contributor.authorLi, Younanen_US
dc.date.issued2012-05-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractFibroblast growth factor (FGF) signaling and downstream activation of the transcription factor ETS1/2 specify the heart cell lineage in the basal chordate Ciona intestinalis. Based on our previous work, 144 candidate FGF/ETS1/2 dependent heart genes have been identified from the Ciona genome. The expression of a large subset of these genes has been verified in the heart precursor lineage using in-situ techniques. Using an bio-informatic approach, we identified candidate enhancers for some of these target genes. We then tested these predicted enhancers through reporter analysis. Our results indicate that the Ets1/2 binding site GGAW and the co-motif ATTA are required for heart lineage enhancer activity. This work can help us understand the conserved, primary role of FGF/Ets in chordate heart lineage specification.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineMolecular and Cellular Biologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
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