Persistent Link:
http://hdl.handle.net/10150/243969
Title:
Early FGF/Ets1/2 Target Heart Genes in Ciona intestinalis
Author:
Jemmett, Jessica L.
Issue Date:
May-2012
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Activation of the transcription factor Ets1/2 through FGF signaling is known to specify heart precursor fate in Ciona intestinalis. In previous research, we identified candidate target genes of Ets1/2 through microarray analysis. Through in situ hybridization assays we have identified a subset of these candidate genes that are expressed specifically in the heart precursor cells immediately following their specification. To find the enhancers for the regulation of these presumed Ets target genes, we are employing bioinformatics to find conserved areas of DNA in the upstream non‐coding DNA between Ciona intestinalis and Ciona savignyi. This analysis will be used to guide ongoing efforts to clone and test predicted enhancer regions using reporter constructs. In depth analysis of identified enhancers will be used to find transcription‐binding sites for Ets and identify co-transcription factors presumed to act in concert with Ets to drive heart precursor cell specification.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Biochemistry and Molecular Biophysics
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleEarly FGF/Ets1/2 Target Heart Genes in Ciona intestinalisen_US
dc.creatorJemmett, Jessica L.en_US
dc.contributor.authorJemmett, Jessica L.en_US
dc.date.issued2012-05-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractActivation of the transcription factor Ets1/2 through FGF signaling is known to specify heart precursor fate in Ciona intestinalis. In previous research, we identified candidate target genes of Ets1/2 through microarray analysis. Through in situ hybridization assays we have identified a subset of these candidate genes that are expressed specifically in the heart precursor cells immediately following their specification. To find the enhancers for the regulation of these presumed Ets target genes, we are employing bioinformatics to find conserved areas of DNA in the upstream non‐coding DNA between Ciona intestinalis and Ciona savignyi. This analysis will be used to guide ongoing efforts to clone and test predicted enhancer regions using reporter constructs. In depth analysis of identified enhancers will be used to find transcription‐binding sites for Ets and identify co-transcription factors presumed to act in concert with Ets to drive heart precursor cell specification.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineBiochemistry and Molecular Biophysicsen_US
thesis.degree.grantorUniversity of Arizonaen_US
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