Neuronal Injury Secondary to Intrathecal Methotrexate in a Rat Model

Persistent Link:
http://hdl.handle.net/10150/243967
Title:
Neuronal Injury Secondary to Intrathecal Methotrexate in a Rat Model
Author:
Humphrey, Ashley Marie
Issue Date:
May-2012
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The purpose of this study was to determine if intrathecally administered methotrexate causes neuronal injury in the cortex of the brain in a rat model. This study was designed to mimic methotrexate treatment in children with acute lymphoblastic leukemia (ALL). Current treatment includes intrathecal chemotherapy, which is administered directly into the central nervous system. While this has significantly improved survival rates, late neurocognitive deficits have been observed. Six Fischer 344 rats were treated with either 4mg/kg methotrexate or artificial cerebral spinal fluid as controls. After five days the rats were sacrificed, brains were sliced into 5μm sections and stained for H and E. Images were taken of the superior, medial, and inferior areas of both sides of the cortex at 40x magnification. Neurons were counted using the ImageJ program (National Institutes of Health). Statistical significance (p<0.05) was found in the medial areas as hypothesized, with the percentage of healthy neurons greater in the control (mean ± SD = 71.7 ± 21.8) versus treatment (55.6 ± 32.9) rats. Although statistical significance (p<0.05) was obtained, contrary to the hypothesis the percentage of healthy neurons was greater in the treatment (66.1 ± 25.0) versus control (51.7 ± 34.1) rats in the inferior areas.
Type:
text; Electronic Thesis
Degree Name:
B.S.N.
Degree Level:
bachelors
Degree Program:
Honors College; Nursing
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleNeuronal Injury Secondary to Intrathecal Methotrexate in a Rat Modelen_US
dc.creatorHumphrey, Ashley Marieen_US
dc.contributor.authorHumphrey, Ashley Marieen_US
dc.date.issued2012-05-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe purpose of this study was to determine if intrathecally administered methotrexate causes neuronal injury in the cortex of the brain in a rat model. This study was designed to mimic methotrexate treatment in children with acute lymphoblastic leukemia (ALL). Current treatment includes intrathecal chemotherapy, which is administered directly into the central nervous system. While this has significantly improved survival rates, late neurocognitive deficits have been observed. Six Fischer 344 rats were treated with either 4mg/kg methotrexate or artificial cerebral spinal fluid as controls. After five days the rats were sacrificed, brains were sliced into 5μm sections and stained for H and E. Images were taken of the superior, medial, and inferior areas of both sides of the cortex at 40x magnification. Neurons were counted using the ImageJ program (National Institutes of Health). Statistical significance (p<0.05) was found in the medial areas as hypothesized, with the percentage of healthy neurons greater in the control (mean ± SD = 71.7 ± 21.8) versus treatment (55.6 ± 32.9) rats. Although statistical significance (p<0.05) was obtained, contrary to the hypothesis the percentage of healthy neurons was greater in the treatment (66.1 ± 25.0) versus control (51.7 ± 34.1) rats in the inferior areas.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.N.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineNursingen_US
thesis.degree.grantorUniversity of Arizonaen_US
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