Schwann Cells Promote Tumor Cell Invasion Through Regulation of the Laminin Receptor α6β1

Persistent Link:
http://hdl.handle.net/10150/243882
Title:
Schwann Cells Promote Tumor Cell Invasion Through Regulation of the Laminin Receptor α6β1
Author:
Chopra, Harsharon; Cress, Anne E.; Sroka, Isis C.
Issue Date:
May-2012
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Neurotropic cancers including pancreatic and prostate cancer utilize nerves as a major route of metastasis in a process known as nerve invasion (NI). During NI, tumor cells invade the perineurium of nerves and into the endoneural space where tumor cells contact Schwann cells responsible for secretion of laminin extracellular matrix proteins and myelination of peripheral nerve axons. The laminin binding cell adhesion receptor α6β1 is expressed on tumor cells undergoing NI and cleavage of α6β1 by urokinase plasminogen activator to form the variant α6β1 promotes tumor cell invasion. Here, we demonstrate that conditioned media from immortalized S16 and S16Y Schwann cells, which are of the myelinating and non-myelinating morphology respectively, regulate α6pβ1 formation and tumor invasion of neurotropic tumor cell lines. The results demonstrate that S16Y conditioned medium inhibited invasion and 􀄮6 conversion to α6p in DU145, CFPAC1 and PC3 cancer cells while S16 conditioned medium increased α6p expression and tumor cell invasion. The increased invasion mediated by the S16 cells was dependent on α6pβ1 since a function-blocking antibody, targeting α6pβ1 formation inhibited invasion. Taken together these results suggest that myelinating Schwann cells in the nerve environment promote α6β1 receptor dependent tumor cell nerve invasion.
Type:
text; Electronic Thesis
Degree Name:
B.S.H.S.
Degree Level:
bachelors
Degree Program:
Honors College; Physiology
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleSchwann Cells Promote Tumor Cell Invasion Through Regulation of the Laminin Receptor α6β1en_US
dc.creatorChopra, Harsharonen_US
dc.contributor.authorChopra, Harsharonen_US
dc.contributor.authorCress, Anne E.en_US
dc.contributor.authorSroka, Isis C.en_US
dc.date.issued2012-05-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractNeurotropic cancers including pancreatic and prostate cancer utilize nerves as a major route of metastasis in a process known as nerve invasion (NI). During NI, tumor cells invade the perineurium of nerves and into the endoneural space where tumor cells contact Schwann cells responsible for secretion of laminin extracellular matrix proteins and myelination of peripheral nerve axons. The laminin binding cell adhesion receptor α6β1 is expressed on tumor cells undergoing NI and cleavage of α6β1 by urokinase plasminogen activator to form the variant α6β1 promotes tumor cell invasion. Here, we demonstrate that conditioned media from immortalized S16 and S16Y Schwann cells, which are of the myelinating and non-myelinating morphology respectively, regulate α6pβ1 formation and tumor invasion of neurotropic tumor cell lines. The results demonstrate that S16Y conditioned medium inhibited invasion and 􀄮6 conversion to α6p in DU145, CFPAC1 and PC3 cancer cells while S16 conditioned medium increased α6p expression and tumor cell invasion. The increased invasion mediated by the S16 cells was dependent on α6pβ1 since a function-blocking antibody, targeting α6pβ1 formation inhibited invasion. Taken together these results suggest that myelinating Schwann cells in the nerve environment promote α6β1 receptor dependent tumor cell nerve invasion.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.H.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplinePhysiologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
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