Opioid and Non-Opoid Actions of Dynorphin A on Spinal Wide Dynamic Range Neurons

Persistent Link:
http://hdl.handle.net/10150/243876
Title:
Opioid and Non-Opoid Actions of Dynorphin A on Spinal Wide Dynamic Range Neurons
Author:
Cai, Alice
Issue Date:
May-2012
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Dynorphin A is an endogenous peptide that can activate opioid receptors and bradykinin receptors; the latter has been proposed to be a mechanism of dynorphin A’s pronociceptive actions. Sensitization of wide dynamic range (WDR) neurons in the spinal dorsal horn causes innocuous stimuli to be perceived as pain. Under conditions of chronic inflammation, elevated levels of dynorphin A are found in he spinal cord and could contribute to central sensitization by modulating WDR neuron function. To test the effect of dynorphin A on WDR neurons, we performed in vivo extracellular recordings on the spinal cord of anesthetized rats in the presence of a dose range of an opioid, dynorphin A (1-13), or non-opioid dynorphin A (2-13). WDR neurons’ response was attenuated in naïve rats by dynorphin A (1-13). Dynorphin A (2-13) did not potentiate WDR response to nociceptive inputs in naive rats. These findings suggest that the pronociceptive actions of intrathecal dynorphin A is unlikely to be mediated by activation of the WDR neurons in physiological conditions. A fragment of dynorphin A, LYS1044, retains high affinity for bradykinin receptors but has no agonist activity, and could provide a basis for therapeutics using dynorphin A as a new modality for pain.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Biochemistry and Molecular Biophysics
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleOpioid and Non-Opoid Actions of Dynorphin A on Spinal Wide Dynamic Range Neuronsen_US
dc.creatorCai, Aliceen_US
dc.contributor.authorCai, Aliceen_US
dc.date.issued2012-05-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractDynorphin A is an endogenous peptide that can activate opioid receptors and bradykinin receptors; the latter has been proposed to be a mechanism of dynorphin A’s pronociceptive actions. Sensitization of wide dynamic range (WDR) neurons in the spinal dorsal horn causes innocuous stimuli to be perceived as pain. Under conditions of chronic inflammation, elevated levels of dynorphin A are found in he spinal cord and could contribute to central sensitization by modulating WDR neuron function. To test the effect of dynorphin A on WDR neurons, we performed in vivo extracellular recordings on the spinal cord of anesthetized rats in the presence of a dose range of an opioid, dynorphin A (1-13), or non-opioid dynorphin A (2-13). WDR neurons’ response was attenuated in naïve rats by dynorphin A (1-13). Dynorphin A (2-13) did not potentiate WDR response to nociceptive inputs in naive rats. These findings suggest that the pronociceptive actions of intrathecal dynorphin A is unlikely to be mediated by activation of the WDR neurons in physiological conditions. A fragment of dynorphin A, LYS1044, retains high affinity for bradykinin receptors but has no agonist activity, and could provide a basis for therapeutics using dynorphin A as a new modality for pain.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineBiochemistry and Molecular Biophysicsen_US
thesis.degree.grantorUniversity of Arizonaen_US
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