Vasopressin Alters the Expression of Aquaporins, Urea Transporters, and ER Stress Genes in the Mouse Renal Medulla

Persistent Link:
http://hdl.handle.net/10150/243731
Title:
Vasopressin Alters the Expression of Aquaporins, Urea Transporters, and ER Stress Genes in the Mouse Renal Medulla
Author:
Adun, Eseoghene
Issue Date:
May-2012
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Vasopressin, an antidiuretic hormone (ADH), plays an essential role in water regulation during dehydration. The purpose of this study is to examine novel genes that may be activated by vasopressin and evaluate the relative expression of several proteins in the renal collecting duct. 48 hours after water restriction measurements confirmed the increased expression of mRNA of AQP2, AQP3 and the ER stress genes AR, ATF3, and GRP78 .We found that water restriction increases the expression of AQP2 and AQP3 proteins to 1.88 ± 0.08 fold vs. control 1.00 ± 0.14 fold and 2.88 ± 0.33 fold vs. control 1.00 ± 0.16 fold, respectively. Water restriction increases the expression of AR and ATF3 proteins to 1.33 ± 0.14 fold vs. control 1.00 ± 0.13 fold and 3.81 ± 0.24 fold vs. control 1.00 ± 0.06 fold, respectively. There was an increased mRNA expression of GRP78 with 1.43 ± 0.11 fold vs. control 1.00 ± 0.04 fold. Vasopressin implantation increases in the mRNA abundance of AQP2 and AQP3 proteins to 1.88 ± 0.09 fold vs. control 1.00 ± 0.07 fold and 1.46 ± 0.04 fold vs. control 1.00 ± 0.15 fold, respectively. Vasopressin implantation increases in the mRNA abundance of UTA-1 and UTA-3 proteins to 1.88 ± 0.11 fold vs. control 1.00 ± 0.02 fold 1.94 ± 0.4 fold vs. control 1.00 ± 0.2 fold, respectively. There was a significant increase in the mRNA abundance of ATF3 2.75 ± 0.55 4 fold vs. control 1.00 ± 0.31 fold as well as CHOP10 to 1.7 ± 0. 4 fold vs. control 1.00 ± 0.09 fold in the renal medulla. Insights on these genes are important as vasopressin levels are high in diseases such as diabetes and congestive heart failure.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Biology
Degree Grantor:
University of Arizona

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleVasopressin Alters the Expression of Aquaporins, Urea Transporters, and ER Stress Genes in the Mouse Renal Medullaen_US
dc.creatorAdun, Eseogheneen_US
dc.contributor.authorAdun, Eseogheneen_US
dc.date.issued2012-05-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractVasopressin, an antidiuretic hormone (ADH), plays an essential role in water regulation during dehydration. The purpose of this study is to examine novel genes that may be activated by vasopressin and evaluate the relative expression of several proteins in the renal collecting duct. 48 hours after water restriction measurements confirmed the increased expression of mRNA of AQP2, AQP3 and the ER stress genes AR, ATF3, and GRP78 .We found that water restriction increases the expression of AQP2 and AQP3 proteins to 1.88 ± 0.08 fold vs. control 1.00 ± 0.14 fold and 2.88 ± 0.33 fold vs. control 1.00 ± 0.16 fold, respectively. Water restriction increases the expression of AR and ATF3 proteins to 1.33 ± 0.14 fold vs. control 1.00 ± 0.13 fold and 3.81 ± 0.24 fold vs. control 1.00 ± 0.06 fold, respectively. There was an increased mRNA expression of GRP78 with 1.43 ± 0.11 fold vs. control 1.00 ± 0.04 fold. Vasopressin implantation increases in the mRNA abundance of AQP2 and AQP3 proteins to 1.88 ± 0.09 fold vs. control 1.00 ± 0.07 fold and 1.46 ± 0.04 fold vs. control 1.00 ± 0.15 fold, respectively. Vasopressin implantation increases in the mRNA abundance of UTA-1 and UTA-3 proteins to 1.88 ± 0.11 fold vs. control 1.00 ± 0.02 fold 1.94 ± 0.4 fold vs. control 1.00 ± 0.2 fold, respectively. There was a significant increase in the mRNA abundance of ATF3 2.75 ± 0.55 4 fold vs. control 1.00 ± 0.31 fold as well as CHOP10 to 1.7 ± 0. 4 fold vs. control 1.00 ± 0.09 fold in the renal medulla. Insights on these genes are important as vasopressin levels are high in diseases such as diabetes and congestive heart failure.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.nameB.S.en_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineBiologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
All Items in UA Campus Repository are protected by copyright, with all rights reserved, unless otherwise indicated.