Persistent Link:
http://hdl.handle.net/10150/228469
Title:
Stop Codon Polymorphism in Two Saccharomyces Species
Author:
Levine, Joshua
Issue Date:
2012
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The origin of new coding sequence is a major puzzle in biology. The evolutionary pressures on new sequences are largely unknown, but structural constraints are thought to play a role. Previously, 3' untranslated region (UTR) conversion to open reading frame (ORF) was observed in Saccharomyces. We sought to identify genes that were polymorphic for stop codon position in S. cerevisiae and S. paradoxus. Using strain sequence data from the Saccharomyces Genome Resequencing Project, we found 1336 genes that had evidence of stop codon polymorphism. Of those, we found 62 genes that had evidence of addition to ancestral sequence that represented the conversion of ancestral 3' UTR to derived ORF. Two of these genes, YGL058W and YNL034W-A, are prime candidates for structural studies as they are short proteins with long additions and known structures. In future studies, they will be used to infer any structural constraints on newly evolving proteins.
Type:
text; Electronic Thesis
Keywords:
Saccharomyces; Stop Codon; Ecology & Evolutionary Biology; Genomics; Novel Proteins
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Ecology & Evolutionary Biology
Degree Grantor:
University of Arizona
Advisor:
Masel, Joanna

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleStop Codon Polymorphism in Two Saccharomyces Speciesen_US
dc.creatorLevine, Joshuaen_US
dc.contributor.authorLevine, Joshuaen_US
dc.date.issued2012-
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe origin of new coding sequence is a major puzzle in biology. The evolutionary pressures on new sequences are largely unknown, but structural constraints are thought to play a role. Previously, 3' untranslated region (UTR) conversion to open reading frame (ORF) was observed in Saccharomyces. We sought to identify genes that were polymorphic for stop codon position in S. cerevisiae and S. paradoxus. Using strain sequence data from the Saccharomyces Genome Resequencing Project, we found 1336 genes that had evidence of stop codon polymorphism. Of those, we found 62 genes that had evidence of addition to ancestral sequence that represented the conversion of ancestral 3' UTR to derived ORF. Two of these genes, YGL058W and YNL034W-A, are prime candidates for structural studies as they are short proteins with long additions and known structures. In future studies, they will be used to infer any structural constraints on newly evolving proteins.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
dc.subjectSaccharomycesen_US
dc.subjectStop Codonen_US
dc.subjectEcology & Evolutionary Biologyen_US
dc.subjectGenomicsen_US
dc.subjectNovel Proteinsen_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineEcology & Evolutionary Biologyen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorMasel, Joannaen_US
dc.contributor.committeememberWalsh, J. B.en_US
dc.contributor.committeememberSanderson, Michaelen_US
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