Persistent Link:
http://hdl.handle.net/10150/220516
Title:
Mycoplasma-like Organisms as the Causal Agent for Macrophylla Decline
Author:
Taylor, Kathryn; Ellis, Danielle
Issue Date:
Sep-1996
Publisher:
College of Agriculture, University of Arizona (Tucson, AZ)
Journal:
Citrus Research Report
Abstract:
Previous literature concerning citrus and other tree crops led us to ask if there was molecular evidence for mycoplasma -like organisms (MLOs) as the causal agent of Macrophylla decline and two other decline diseases, citrus blight and lemon sieve tube necrosis. We had molecular probes available to us that were either specific for MLOs of tree diseases and others that were universal for all known types of MLOs. We used a polymerase chain reaction (MLO) to determine if MLOs were present in the vascular tissues of decline and healthy citrus. I all trials performed, the trees were negative for MLO-PCR products. In addition, we attempted to transmit putative MLO 's from decline affected trees to Vinca rosea MLO-nurse plants. We were unable to affect this type of transfer. In addition, our attempts to identify MLO's in phloem tissue gave us negative results. We have since revised our hypothesis. We are currently pursuing the hypothesis that these decline disorders are the result of a rootstock scion incompatibility, that we may be able to avoid culturally, while maintaining these valuable combinations.
Keywords:
Agriculture -- Arizona; Citrus fruits -- Arizona; Citrus fruits -- Diseases
Series/Report no.:
Series P-105; 370105
Sponsors:
Citrus Research Council

Full metadata record

DC FieldValue Language
dc.titleMycoplasma-like Organisms as the Causal Agent for Macrophylla Declineen_US
dc.contributor.authorTaylor, Kathrynen_US
dc.contributor.authorEllis, Danielleen_US
dc.date.issued1996-09-
dc.publisherCollege of Agriculture, University of Arizona (Tucson, AZ)en_US
dc.identifier.journalCitrus Research Reporten_US
dc.description.abstractPrevious literature concerning citrus and other tree crops led us to ask if there was molecular evidence for mycoplasma -like organisms (MLOs) as the causal agent of Macrophylla decline and two other decline diseases, citrus blight and lemon sieve tube necrosis. We had molecular probes available to us that were either specific for MLOs of tree diseases and others that were universal for all known types of MLOs. We used a polymerase chain reaction (MLO) to determine if MLOs were present in the vascular tissues of decline and healthy citrus. I all trials performed, the trees were negative for MLO-PCR products. In addition, we attempted to transmit putative MLO 's from decline affected trees to Vinca rosea MLO-nurse plants. We were unable to affect this type of transfer. In addition, our attempts to identify MLO's in phloem tissue gave us negative results. We have since revised our hypothesis. We are currently pursuing the hypothesis that these decline disorders are the result of a rootstock scion incompatibility, that we may be able to avoid culturally, while maintaining these valuable combinations.en_US
dc.subjectAgriculture -- Arizonaen_US
dc.subjectCitrus fruits -- Arizonaen_US
dc.subjectCitrus fruits -- Diseasesen_US
dc.identifier.urihttp://hdl.handle.net/10150/220516-
dc.relation.ispartofseriesSeries P-105en_US
dc.relation.ispartofseries370105en_US
dc.description.sponsorshipCitrus Research Councilen_US
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