Persistent Link:
http://hdl.handle.net/10150/197701
Title:
Transgenic Comparisons of Pink Bollworm Efficacy and Response to Heat Stress
Author:
Ellsworth, Peter C.; Moser, Hal; Henneberry, Tom; Majeau, Ghislane; Subramani, Jay
Affiliation:
Maricopa Agricultural Center & Department of Entomology, University of Arizona, Maricopa, AZ; USDA-ARS, Western Cotton Research Laboratory
Issue Date:
Jun-2002
Publisher:
College of Agriculture, University of Arizona (Tucson, AZ)
Journal:
Cotton: A College of Agriculture and Life Sciences Report
Abstract:
Fifteen lines from 3 different cotton families were compared. Each family had a conventional, non-transgenic standard, as well as 4 other transgenic lines. In some cases, near isogenic lines were available that theoretically only vary from their sibling lines in the presence or absence of one or more transgenes. Each Bt line was evaluated for this trait’s efficacy in controlling pink bollworm under high pressure, artificial infestations. Various agronomic properties were measured including yield, micronaire, ginning properties, and fiber quality. Heat tolerance, a key goal for Arizona adapted varieties, was also evaluated using a flower rating system. The Cry1Ac gene performed flawlessly in preventing PBW larval development when expressed alone (Bollgard®) or in combination with Cry2Ab (Bollgard II®) (i.e., 100% effective, 0 large larvae from 30185 PBW entry holes). In all cases where large larvae were found in Bollgard or Bollgard II plots, the plants bearing the infested bolls were not expressing the Cry1Ac toxin. Thus, those few times when larvae were found, it was due to contaminants in the seed supply. The novel Cry2Ab only expressing plants, produced for non-commercial testing purposes, were also very effective in controlling PBW large larval development; however, control was less than the Cry1Ac-expressing lines (99.622%, 3 large larvae from 4436 entry holes). The ramifications of this are discussed. In terms of agronomic performance, the transgenic lines performed similarly within families and usually not different from the conventional standards. In some cases, statistically different results were found; however, in all but a few cases, performance parameters were superior in the transgenic lines when compared to the conventional standard. Even so, there are instances where characteristics of the transgenic line were inferior to the conventional standard, especially in some fiber properties. Heat tolerance was again similar throughout 2 of the cotton families (SG125 and DP50). However, for the DP5415 family, 3 of the 4 transgenic lines outperformed the conventional standard. More testing under more environmental conditions is warranted before firm conclusions are drawn.
Keywords:
Agriculture -- Arizona; Cotton -- Arizona; Insect investigations
Series/Report no.:
AZ1283; Series P-130

Full metadata record

DC FieldValue Language
dc.titleTransgenic Comparisons of Pink Bollworm Efficacy and Response to Heat Stressen_US
dc.contributor.authorEllsworth, Peter C.en_US
dc.contributor.authorMoser, Halen_US
dc.contributor.authorHenneberry, Tomen_US
dc.contributor.authorMajeau, Ghislaneen_US
dc.contributor.authorSubramani, Jayen_US
dc.contributor.departmentMaricopa Agricultural Center & Department of Entomology, University of Arizona, Maricopa, AZen_US
dc.contributor.departmentUSDA-ARS, Western Cotton Research Laboratoryen_US
dc.date.issued2002-06-
dc.publisherCollege of Agriculture, University of Arizona (Tucson, AZ)en_US
dc.identifier.journalCotton: A College of Agriculture and Life Sciences Reporten_US
dc.description.abstractFifteen lines from 3 different cotton families were compared. Each family had a conventional, non-transgenic standard, as well as 4 other transgenic lines. In some cases, near isogenic lines were available that theoretically only vary from their sibling lines in the presence or absence of one or more transgenes. Each Bt line was evaluated for this trait’s efficacy in controlling pink bollworm under high pressure, artificial infestations. Various agronomic properties were measured including yield, micronaire, ginning properties, and fiber quality. Heat tolerance, a key goal for Arizona adapted varieties, was also evaluated using a flower rating system. The Cry1Ac gene performed flawlessly in preventing PBW larval development when expressed alone (Bollgard®) or in combination with Cry2Ab (Bollgard II®) (i.e., 100% effective, 0 large larvae from 30185 PBW entry holes). In all cases where large larvae were found in Bollgard or Bollgard II plots, the plants bearing the infested bolls were not expressing the Cry1Ac toxin. Thus, those few times when larvae were found, it was due to contaminants in the seed supply. The novel Cry2Ab only expressing plants, produced for non-commercial testing purposes, were also very effective in controlling PBW large larval development; however, control was less than the Cry1Ac-expressing lines (99.622%, 3 large larvae from 4436 entry holes). The ramifications of this are discussed. In terms of agronomic performance, the transgenic lines performed similarly within families and usually not different from the conventional standards. In some cases, statistically different results were found; however, in all but a few cases, performance parameters were superior in the transgenic lines when compared to the conventional standard. Even so, there are instances where characteristics of the transgenic line were inferior to the conventional standard, especially in some fiber properties. Heat tolerance was again similar throughout 2 of the cotton families (SG125 and DP50). However, for the DP5415 family, 3 of the 4 transgenic lines outperformed the conventional standard. More testing under more environmental conditions is warranted before firm conclusions are drawn.en_US
dc.subjectAgriculture -- Arizonaen_US
dc.subjectCotton -- Arizonaen_US
dc.subjectInsect investigationsen_US
dc.identifier.urihttp://hdl.handle.net/10150/197701-
dc.relation.ispartofseriesAZ1283en_US
dc.relation.ispartofseriesSeries P-130-
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