The Influence of Omega-3 Fatty Acid Supplementation on Stallion Spermatozoa Survival Following Short- and Long-Term Preservation

Persistent Link:
http://hdl.handle.net/10150/195991
Title:
The Influence of Omega-3 Fatty Acid Supplementation on Stallion Spermatozoa Survival Following Short- and Long-Term Preservation
Author:
Harris, Mary Ann
Issue Date:
2005
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Study objectives were to; 1) determine if supplementing of n-3 fatty acids improves membrane integrity, and hence viability and motility of stallion spermatozoa following cold storage, and following cryopreservation, and 2) determine if n-3 supplementation alters the fatty acid composition of stallion spermatozoa. Data indicate that following 90 d of n-3 supplementation daily sperm output and the percentage of morphologically normal sperm in neat semen are increased. Omega-3 supplementation for 90 d did not improve spermatozoal motility or viability following short-term preservation (0, 24 h, 48 h), or following cryopreservation. Although motility was unchanged in this study, individual stallion responses did indicate that n-3 supplementation in stallions with marginal to poor semen quality may benefit from n-3 supplementation. Finally, n-3 fatty acid supplementation does alter plasmalemma fatty acid composition. Spermatozoa from supplemented stallions had increased docosahexaenoic acid (DHA) concentrations as compared to non-supplemented stallions. It is postulated that an increase in long chain n-3 fatty acids, specifically DHA in spermatozoa membrane improves membrane integrity, and thus enhances spermatozoa recovery following the stresses of cold storage and cryopreservation. This phenomenon appears to be beneficial to stallions with marginal to poor quality ejaculates.
Type:
text; Electronic Dissertation
Keywords:
Stallion; spermatozoa; DHA; n-3
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Animal Sciences; Graduate College
Degree Grantor:
University of Arizona
Committee Chair:
Arns, Mark J.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleThe Influence of Omega-3 Fatty Acid Supplementation on Stallion Spermatozoa Survival Following Short- and Long-Term Preservationen_US
dc.creatorHarris, Mary Annen_US
dc.contributor.authorHarris, Mary Annen_US
dc.date.issued2005en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractStudy objectives were to; 1) determine if supplementing of n-3 fatty acids improves membrane integrity, and hence viability and motility of stallion spermatozoa following cold storage, and following cryopreservation, and 2) determine if n-3 supplementation alters the fatty acid composition of stallion spermatozoa. Data indicate that following 90 d of n-3 supplementation daily sperm output and the percentage of morphologically normal sperm in neat semen are increased. Omega-3 supplementation for 90 d did not improve spermatozoal motility or viability following short-term preservation (0, 24 h, 48 h), or following cryopreservation. Although motility was unchanged in this study, individual stallion responses did indicate that n-3 supplementation in stallions with marginal to poor semen quality may benefit from n-3 supplementation. Finally, n-3 fatty acid supplementation does alter plasmalemma fatty acid composition. Spermatozoa from supplemented stallions had increased docosahexaenoic acid (DHA) concentrations as compared to non-supplemented stallions. It is postulated that an increase in long chain n-3 fatty acids, specifically DHA in spermatozoa membrane improves membrane integrity, and thus enhances spermatozoa recovery following the stresses of cold storage and cryopreservation. This phenomenon appears to be beneficial to stallions with marginal to poor quality ejaculates.en_US
dc.typetexten_US
dc.typeElectronic Dissertationen_US
dc.subjectStallionen_US
dc.subjectspermatozoaen_US
dc.subjectDHAen_US
dc.subjectn-3en_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineAnimal Sciencesen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.chairArns, Mark J.en_US
dc.contributor.committeememberAllen, Ronald E.en_US
dc.contributor.committeememberBaumgard, Lance H.en_US
dc.contributor.committeememberRaub, Randel H.en_US
dc.contributor.committeememberSchurg, William A.en_US
dc.identifier.proquest1389en_US
dc.identifier.oclc137355404en_US
All Items in UA Campus Repository are protected by copyright, with all rights reserved, unless otherwise indicated.