The Effects of EIF5A and of the Polyamines on RNA Processing, Translation, and P-Body Formation

Persistent Link:
http://hdl.handle.net/10150/195484
Title:
The Effects of EIF5A and of the Polyamines on RNA Processing, Translation, and P-Body Formation
Author:
Childs, April Celeste
Issue Date:
2005
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The polyamines positively charged molecules that are able to affect nucleic acid structure. Investigation of polyamine and RNA interaction shows that the two are able to form aggregates and these aggregates may be responsible for the inhibition of RNA decapping that is seen in the presence of polyamines. The polyamines are also responsible for a post-translational modification of a protein, eukaryotic initiation factor 5a (eIF5A). This protein is known to affect translation and RNA decay. This investigation shows that eIF5A is able to affect the formation of foci by Dhh1 but does not affect DCP1 or DCP2 foci formation. This investigation also shows that eIF5A mutation leads to a depression of polysome profiles and 35S-met incorporation and therefore eIF5A may truly be a translation initiation factor. This study also describes unique interactions of eIF5A with Dhh1p and eIF5A-independent effects of the polyamines on gene expression. The inhibition of eIF5A's hypusine modification leads to an increase in phoshorylation of eIF2α and this may contribute the induction of apoptosis and inhibition of protein synthesis associated with inhibition of eIF5A's hypusine modification.
Type:
text; Electronic Dissertation
Keywords:
Molecular & Cellular Biology
Degree Name:
PhD
Degree Level:
doctoral
Degree Program:
Molecular & Cellular Biology; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Gerner, Eugene W.
Committee Chair:
Gerner, Eugene W.

Full metadata record

DC FieldValue Language
dc.language.isoENen_US
dc.titleThe Effects of EIF5A and of the Polyamines on RNA Processing, Translation, and P-Body Formationen_US
dc.creatorChilds, April Celesteen_US
dc.contributor.authorChilds, April Celesteen_US
dc.date.issued2005en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe polyamines positively charged molecules that are able to affect nucleic acid structure. Investigation of polyamine and RNA interaction shows that the two are able to form aggregates and these aggregates may be responsible for the inhibition of RNA decapping that is seen in the presence of polyamines. The polyamines are also responsible for a post-translational modification of a protein, eukaryotic initiation factor 5a (eIF5A). This protein is known to affect translation and RNA decay. This investigation shows that eIF5A is able to affect the formation of foci by Dhh1 but does not affect DCP1 or DCP2 foci formation. This investigation also shows that eIF5A mutation leads to a depression of polysome profiles and 35S-met incorporation and therefore eIF5A may truly be a translation initiation factor. This study also describes unique interactions of eIF5A with Dhh1p and eIF5A-independent effects of the polyamines on gene expression. The inhibition of eIF5A's hypusine modification leads to an increase in phoshorylation of eIF2α and this may contribute the induction of apoptosis and inhibition of protein synthesis associated with inhibition of eIF5A's hypusine modification.en_US
dc.typetexten_US
dc.typeElectronic Dissertationen_US
dc.subjectMolecular & Cellular Biologyen_US
thesis.degree.namePhDen_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineMolecular & Cellular Biologyen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorGerner, Eugene W.en_US
dc.contributor.chairGerner, Eugene W.en_US
dc.contributor.committeememberParker, Roy R.en_US
dc.contributor.committeememberBowden, G. Timen_US
dc.contributor.committeememberCress, Anneen_US
dc.contributor.committeememberMartinez, Jesseen_US
dc.identifier.proquest1326en_US
dc.identifier.oclc137355035en_US
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