Study of microtubule templates for fabrication of nano-interconnects

Persistent Link:
http://hdl.handle.net/10150/195241
Title:
Study of microtubule templates for fabrication of nano-interconnects
Author:
Yang, Yi
Issue Date:
2005
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Microtubules (MTs), whose basic units are a and ß tubulin proteins, are self-assembled proteinaceous filaments with nanometer scale diameters and micrometer scale lengths. Their aspect ratio, directionality, the reversibility of their assembly and their ability to be metallized by electroless plating make them good candidates to serve as templates for the fabrication of nanowires and other nanoscale devices. In addition, tubulin proteins can provide biological interactions with a naturally high specificity.Toward the goal of manufacturing MT-based metallic nanowires and networks of nanowires on a silicon wafer, I studied the influence of pH, temperature, and several biomolecules on the stability of MTs in solutions, as well as the surface effect on the dynamics of disassembly of microtubules. Secondly, I demonstrated the metallization of MTs by electroless nickel plating both in solution and on hydrophilic oxidized Si surface. After being activated by Pt, nickel coated MT surfaces during the electroless plating, with a thickness of several nanometers. Due to the different kinetics of the process, MTs metallized on the oxidized Si wafer are slightly different from MTs metallized in solutions. Finally, we explored controlled nucleation and growth of microtubules directly from a collection of g-tubulin monomers. g-tubulins bind to modified gold electrodes on a silicon wafer through an organic linker, Glutathione s-transferase, creating a g-tubulin layer for MT growth. MTs unambiguously originated from the surface-bound g-tubulin layer on the gold electrode, proving that the surface-bound g-tubulin retains its biological ability of nucleating MT growth.
Type:
text; Electronic Dissertation
Keywords:
Microtubule; interconnect; bottom-up; nanotechnology
Degree Name:
PhD
Degree Level:
doctoral
Degree Program:
Materials Science & Engineering; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Deymier, Pierre A.
Committee Chair:
Deymier, Pierre A.

Full metadata record

DC FieldValue Language
dc.language.isoENen_US
dc.titleStudy of microtubule templates for fabrication of nano-interconnectsen_US
dc.creatorYang, Yien_US
dc.contributor.authorYang, Yien_US
dc.date.issued2005en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractMicrotubules (MTs), whose basic units are a and ß tubulin proteins, are self-assembled proteinaceous filaments with nanometer scale diameters and micrometer scale lengths. Their aspect ratio, directionality, the reversibility of their assembly and their ability to be metallized by electroless plating make them good candidates to serve as templates for the fabrication of nanowires and other nanoscale devices. In addition, tubulin proteins can provide biological interactions with a naturally high specificity.Toward the goal of manufacturing MT-based metallic nanowires and networks of nanowires on a silicon wafer, I studied the influence of pH, temperature, and several biomolecules on the stability of MTs in solutions, as well as the surface effect on the dynamics of disassembly of microtubules. Secondly, I demonstrated the metallization of MTs by electroless nickel plating both in solution and on hydrophilic oxidized Si surface. After being activated by Pt, nickel coated MT surfaces during the electroless plating, with a thickness of several nanometers. Due to the different kinetics of the process, MTs metallized on the oxidized Si wafer are slightly different from MTs metallized in solutions. Finally, we explored controlled nucleation and growth of microtubules directly from a collection of g-tubulin monomers. g-tubulins bind to modified gold electrodes on a silicon wafer through an organic linker, Glutathione s-transferase, creating a g-tubulin layer for MT growth. MTs unambiguously originated from the surface-bound g-tubulin layer on the gold electrode, proving that the surface-bound g-tubulin retains its biological ability of nucleating MT growth.en_US
dc.typetexten_US
dc.typeElectronic Dissertationen_US
dc.subjectMicrotubuleen_US
dc.subjectinterconnecten_US
dc.subjectbottom-upen_US
dc.subjectnanotechnologyen_US
thesis.degree.namePhDen_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineMaterials Science & Engineeringen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorDeymier, Pierre A.en_US
dc.contributor.chairDeymier, Pierre A.en_US
dc.contributor.committeememberHoying, James B.en_US
dc.contributor.committeememberRaghavan, Srinien_US
dc.identifier.proquest1127en_US
dc.identifier.oclc137354102en_US
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