The Role of Voltage Dependent Calcium Channels in Identified Motoneurons During Fictive Locomotor Behavior

Persistent Link:
http://hdl.handle.net/10150/195192
Title:
The Role of Voltage Dependent Calcium Channels in Identified Motoneurons During Fictive Locomotor Behavior
Author:
Worrell, Jason Walter
Issue Date:
2008
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The primary goal of this work was to examine the role of voltage-dependent Ca2+ channels in regulating the output of larval Drosophila motoneurons functioning within an intact network. To accomplish this goal, two major aims were addressed: 1. To determine whether larval Drosophila motoneurons express voltage-dependent Ca2+ channels in their central processes, and further, to determine the genes responsible. 2. To determine the role of centrally expressed voltage-dependent Ca2+ channels in the regulation of motoneuron output as motoneurons receive behaviorally relevant input from the locomotor network. To address these goals, genetic tools available in Drosophila were used along side in situ patch clamp techniques from larval motoneurons.Using whole cell voltage-clamp techniques in situ, we have shown that two identified motoneurons, aCC and RP-2, carry voltage-dependent currents recorded from the soma. Dmca1D, the L-type like channel in Drosophila, is primarily responsible for this current. Expressing Dmca1D RNAi in aCC and RP-2, as the preparation displayed fictive bouts of locomotion, caused an increase in burst duration in both RP-2 and aCC as well as an increase in the number of action potentials fired per burst. Additionally, the afterhyperpolarization between spikes was greatly reduced and spiking became less regular. This work indicates a role for Dmca1D in the processing of synaptic information in Drosophila motoneurons aCC and RP-2.
Type:
text; Electronic Dissertation
Keywords:
calcium; Drosophila; locomotion; motoneuron; voltage gated
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Physiological Sciences; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Levine, Richard
Committee Chair:
Levine, Richard

Full metadata record

DC FieldValue Language
dc.language.isoENen_US
dc.titleThe Role of Voltage Dependent Calcium Channels in Identified Motoneurons During Fictive Locomotor Behavioren_US
dc.creatorWorrell, Jason Walteren_US
dc.contributor.authorWorrell, Jason Walteren_US
dc.date.issued2008en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe primary goal of this work was to examine the role of voltage-dependent Ca2+ channels in regulating the output of larval Drosophila motoneurons functioning within an intact network. To accomplish this goal, two major aims were addressed: 1. To determine whether larval Drosophila motoneurons express voltage-dependent Ca2+ channels in their central processes, and further, to determine the genes responsible. 2. To determine the role of centrally expressed voltage-dependent Ca2+ channels in the regulation of motoneuron output as motoneurons receive behaviorally relevant input from the locomotor network. To address these goals, genetic tools available in Drosophila were used along side in situ patch clamp techniques from larval motoneurons.Using whole cell voltage-clamp techniques in situ, we have shown that two identified motoneurons, aCC and RP-2, carry voltage-dependent currents recorded from the soma. Dmca1D, the L-type like channel in Drosophila, is primarily responsible for this current. Expressing Dmca1D RNAi in aCC and RP-2, as the preparation displayed fictive bouts of locomotion, caused an increase in burst duration in both RP-2 and aCC as well as an increase in the number of action potentials fired per burst. Additionally, the afterhyperpolarization between spikes was greatly reduced and spiking became less regular. This work indicates a role for Dmca1D in the processing of synaptic information in Drosophila motoneurons aCC and RP-2.en_US
dc.typetexten_US
dc.typeElectronic Dissertationen_US
dc.subjectcalciumen_US
dc.subjectDrosophilaen_US
dc.subjectlocomotionen_US
dc.subjectmotoneuronen_US
dc.subjectvoltage gateden_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplinePhysiological Sciencesen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorLevine, Richarden_US
dc.contributor.chairLevine, Richarden_US
dc.contributor.committeememberDuch, Carstenen_US
dc.contributor.committeememberFuglevand, Andyen_US
dc.contributor.committeememberZinsmaier, Konraden_US
dc.contributor.committeememberGronenberg, Wulfilaen_US
dc.identifier.proquest10204en_US
dc.identifier.oclc659750791en_US
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