ANALYSIS OF THE AMINO-TERMINAL DOMAIN OF DROSOPHILA RBF1 INDICATES NOVEL ROLES IN CELL REGULATION

Persistent Link:
http://hdl.handle.net/10150/194726
Title:
ANALYSIS OF THE AMINO-TERMINAL DOMAIN OF DROSOPHILA RBF1 INDICATES NOVEL ROLES IN CELL REGULATION
Author:
Ahlander, Joseph Andrew
Issue Date:
2009
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The retinoblastoma tumor suppressor protein (RB) is an important regulator of the cell cycle and development. Significantly, RB is inactivated in a majority of human cancers. Thus, elucidating the function of RB will give us a better understanding of how it prevents cancer. Many decades of research have yielded a detailed understanding of the role of RB in cell proliferation through transcriptional repression of target genes. However, the precise mechanisms of its action in many cellular pathways are poorly understood, including the control of DNA replication and post-transcriptional control of gene expression. Drosophila melanogaster presents a simplified genetic system to study cancer genes. Several published observations have suggested a role for RB in regulating DNA replication. Interestingly, other data indicate that RB associates with RNA processing factors. I have characterized novel protein-protein interactions with the Drosophila retinoblastoma tumor suppressor homologue Rbf, with an emphasis on its poorly characterized N-terminal domain. I describe the interaction of Rbf with the origin recognition complex, indicating a unique connection to DNA replication control. I also show that Rbf interacts with the RNA binding protein Squid, and review the literature that suggests potential role of RB/E2F in the control of RNA processing. The ability to control RNA processing may be an additional, unappreciated mode of gene regulation by RB. A focused study of the uncharacterized amino-terminal domain of Rbf has revealed new details about the retinoblastoma tumor suppressor in cell regulation, including DNA replication and RNA processing.
Type:
text; Electronic Dissertation
Keywords:
DNA replication initiation; origin recognition complex; retinoblastoma tumor suppressor; RNA processing
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Molecular & Cellular Biology; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Bosco, Giovanni
Committee Chair:
Bosco, Giovanni

Full metadata record

DC FieldValue Language
dc.language.isoENen_US
dc.titleANALYSIS OF THE AMINO-TERMINAL DOMAIN OF DROSOPHILA RBF1 INDICATES NOVEL ROLES IN CELL REGULATIONen_US
dc.creatorAhlander, Joseph Andrewen_US
dc.contributor.authorAhlander, Joseph Andrewen_US
dc.date.issued2009en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe retinoblastoma tumor suppressor protein (RB) is an important regulator of the cell cycle and development. Significantly, RB is inactivated in a majority of human cancers. Thus, elucidating the function of RB will give us a better understanding of how it prevents cancer. Many decades of research have yielded a detailed understanding of the role of RB in cell proliferation through transcriptional repression of target genes. However, the precise mechanisms of its action in many cellular pathways are poorly understood, including the control of DNA replication and post-transcriptional control of gene expression. Drosophila melanogaster presents a simplified genetic system to study cancer genes. Several published observations have suggested a role for RB in regulating DNA replication. Interestingly, other data indicate that RB associates with RNA processing factors. I have characterized novel protein-protein interactions with the Drosophila retinoblastoma tumor suppressor homologue Rbf, with an emphasis on its poorly characterized N-terminal domain. I describe the interaction of Rbf with the origin recognition complex, indicating a unique connection to DNA replication control. I also show that Rbf interacts with the RNA binding protein Squid, and review the literature that suggests potential role of RB/E2F in the control of RNA processing. The ability to control RNA processing may be an additional, unappreciated mode of gene regulation by RB. A focused study of the uncharacterized amino-terminal domain of Rbf has revealed new details about the retinoblastoma tumor suppressor in cell regulation, including DNA replication and RNA processing.en_US
dc.typetexten_US
dc.typeElectronic Dissertationen_US
dc.subjectDNA replication initiationen_US
dc.subjectorigin recognition complexen_US
dc.subjectretinoblastoma tumor suppressoren_US
dc.subjectRNA processingen_US
thesis.degree.namePh.D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineMolecular & Cellular Biologyen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorBosco, Giovannien_US
dc.contributor.chairBosco, Giovannien_US
dc.contributor.committeememberDixon, Kathleenen_US
dc.contributor.committeememberSchroeder, Joyceen_US
dc.contributor.committeememberMartinez, Jesseen_US
dc.contributor.committeememberZarnescu, Danielaen_US
dc.identifier.proquest10306en_US
dc.identifier.oclc659750926en_US
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