Dynamic Contrast-Enhanced Magnetic Resonance Imaging & Fluorescence Microscopy of Tumor Microvascular Permeability

Persistent Link:
http://hdl.handle.net/10150/193555
Title:
Dynamic Contrast-Enhanced Magnetic Resonance Imaging & Fluorescence Microscopy of Tumor Microvascular Permeability
Author:
Jennings, Dominique Louise
Issue Date:
2008
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Microvascular permeability is a pharmacologic indicator of tumor response to therapy, and it is expected that this biomarker will evolve into a clinical surrogate endpoint and be integrated into protocols for determining patient response to antiangiogenic or antivascular therapies. The goal of this research is to develop a method by which microvascular permeability (Ktrans) and vascular volume (vp) as measured by DCE-MRI were directly compared to the same parameters measured by intravital fluorescence microscopy in an MRI-compatible window chamber model. Dynamic contrast enhanced-MRI (DCE-MRI) is a non-invasive, clinically useful imaging approach that has been used extensively to measure active changes in tumor microvascular hemodynamics. However, uncertainties exist in DCE-MRI as it does not interrogate the contrast reagent (CR) itself, but the effect of the CR on tissue water relaxivity. Thus, direct comparison of DCE-MRI with a more quantitative measure would help better define the derived parameters. The combined imaging system was able to obtain both dynamic contrast-enhanced MRI data high spatio-termporal resolution fluorescence data following injection of fluorescent and gadolinium co-labeled albumin. This approach allowed for the cross-validation of vascular permeability data, in relation tumor growth, angiogenesis and response to therapy in both imaging systems.
Type:
text; Electronic Dissertation
Keywords:
dynamic contrast enhanced-MRI; intravital fluorescence microscopy; microvascular permeability; vascular volume; dorsal midline skinfold window chamber
Degree Name:
PhD
Degree Level:
doctoral
Degree Program:
Biomedical Engineering; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Gillies, Robert J.; Trouard, Theodore P.
Committee Chair:
Gillies, Robert J.; Trouard, Theodore P.

Full metadata record

DC FieldValue Language
dc.language.isoENen_US
dc.titleDynamic Contrast-Enhanced Magnetic Resonance Imaging & Fluorescence Microscopy of Tumor Microvascular Permeabilityen_US
dc.creatorJennings, Dominique Louiseen_US
dc.contributor.authorJennings, Dominique Louiseen_US
dc.date.issued2008en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractMicrovascular permeability is a pharmacologic indicator of tumor response to therapy, and it is expected that this biomarker will evolve into a clinical surrogate endpoint and be integrated into protocols for determining patient response to antiangiogenic or antivascular therapies. The goal of this research is to develop a method by which microvascular permeability (Ktrans) and vascular volume (vp) as measured by DCE-MRI were directly compared to the same parameters measured by intravital fluorescence microscopy in an MRI-compatible window chamber model. Dynamic contrast enhanced-MRI (DCE-MRI) is a non-invasive, clinically useful imaging approach that has been used extensively to measure active changes in tumor microvascular hemodynamics. However, uncertainties exist in DCE-MRI as it does not interrogate the contrast reagent (CR) itself, but the effect of the CR on tissue water relaxivity. Thus, direct comparison of DCE-MRI with a more quantitative measure would help better define the derived parameters. The combined imaging system was able to obtain both dynamic contrast-enhanced MRI data high spatio-termporal resolution fluorescence data following injection of fluorescent and gadolinium co-labeled albumin. This approach allowed for the cross-validation of vascular permeability data, in relation tumor growth, angiogenesis and response to therapy in both imaging systems.en_US
dc.typetexten_US
dc.typeElectronic Dissertationen_US
dc.subjectdynamic contrast enhanced-MRIen_US
dc.subjectintravital fluorescence microscopyen_US
dc.subjectmicrovascular permeabilityen_US
dc.subjectvascular volumeen_US
dc.subjectdorsal midline skinfold window chamberen_US
thesis.degree.namePhDen_US
thesis.degree.leveldoctoralen_US
thesis.degree.disciplineBiomedical Engineeringen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorGillies, Robert J.en_US
dc.contributor.advisorTrouard, Theodore P.en_US
dc.contributor.chairGillies, Robert J.en_US
dc.contributor.chairTrouard, Theodore P.en_US
dc.contributor.committeememberRaghunand, Natarajanen_US
dc.contributor.committeememberGatenby, Robert A.en_US
dc.identifier.proquest2557en_US
dc.identifier.oclc659748486en_US
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