Persistent Link:
http://hdl.handle.net/10150/193457
Title:
Inheritance of Pisatin Demethylase in the Fusaria
Author:
Milani, Nicholas Anthony
Issue Date:
2010
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Upon recognition of the pathogen, plants initiate defense responses that can include the production of antimicrobial compounds such as phytoalexins. Nectria haematococca mating population VI (MPVI) is a filamentous ascomycete that contains a cluster of genes known as the pea pathogenicity (PEP) cluster in which the pisatin demethylase (PDA) gene resides. PDA is responsible for the detoxification of the phytoalexin, pisatin, produced by the pea plant (Pisum sativum). Thus, PDA allows the fungus to colonize and become a pea pathogen. It has been proposed that the evolutionary origin of PDA and the PEP cluster is explained by a horizontal gene transfer (HGT) event. The observations supporting this hypothesis include the location of the PEP cluster on a conditionally dispensable (CD) chromosome, the phylogenetically discontinuous distribution of the cluster among closely related species, and bias in GC content and codon usage. This study used a three-pronged approach to test the hypothesis that PDA and the PEP cluster were inherited via HGT. Percent identities of conserved genes, along with GC content analysis and phylogenetics support vertical inheritance.
Type:
text; Electronic Thesis
Keywords:
HGT; Horizontal; PDA; Pisatin
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Plant Pathology; Graduate College
Degree Grantor:
University of Arizona
Advisor:
VanEtten, Hans D
Committee Chair:
VanEtten, Hans D

Full metadata record

DC FieldValue Language
dc.language.isoENen_US
dc.titleInheritance of Pisatin Demethylase in the Fusariaen_US
dc.creatorMilani, Nicholas Anthonyen_US
dc.contributor.authorMilani, Nicholas Anthonyen_US
dc.date.issued2010en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractUpon recognition of the pathogen, plants initiate defense responses that can include the production of antimicrobial compounds such as phytoalexins. Nectria haematococca mating population VI (MPVI) is a filamentous ascomycete that contains a cluster of genes known as the pea pathogenicity (PEP) cluster in which the pisatin demethylase (PDA) gene resides. PDA is responsible for the detoxification of the phytoalexin, pisatin, produced by the pea plant (Pisum sativum). Thus, PDA allows the fungus to colonize and become a pea pathogen. It has been proposed that the evolutionary origin of PDA and the PEP cluster is explained by a horizontal gene transfer (HGT) event. The observations supporting this hypothesis include the location of the PEP cluster on a conditionally dispensable (CD) chromosome, the phylogenetically discontinuous distribution of the cluster among closely related species, and bias in GC content and codon usage. This study used a three-pronged approach to test the hypothesis that PDA and the PEP cluster were inherited via HGT. Percent identities of conserved genes, along with GC content analysis and phylogenetics support vertical inheritance.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
dc.subjectHGTen_US
dc.subjectHorizontalen_US
dc.subjectPDAen_US
dc.subjectPisatinen_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplinePlant Pathologyen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorVanEtten, Hans Den_US
dc.contributor.chairVanEtten, Hans Den_US
dc.contributor.committeememberArnold, Elizabeth Aen_US
dc.contributor.committeememberRounsley, Steven Den_US
dc.identifier.proquest11054en_US
dc.identifier.oclc659754992en_US
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