ANNOTATION OF WHITEFLY EXPRESSED SEQUENCE TAGS AND VALIDATION OF GENES WITH POTENTIAL SIGNIFICANCE TO BEGOMOVIRUS TRANSMISSION

Persistent Link:
http://hdl.handle.net/10150/193455
Title:
ANNOTATION OF WHITEFLY EXPRESSED SEQUENCE TAGS AND VALIDATION OF GENES WITH POTENTIAL SIGNIFICANCE TO BEGOMOVIRUS TRANSMISSION
Author:
Saripalli, Chandrasekhar
Issue Date:
2008
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The whitefly Bemisia tabaci (Gennadius) (Hemiptera) complex is the sole arthropod vector of the genus, Begomovirus (family, Geminiviridae), which causes debilitating diseases of plants, worldwide. Virus-vector specificity is conferred through co-evolved, whitefly vector-viral capsid protein-protein interactions. Membrane-bound receptors are thought to facilitate virion passage across the gut-hemolymph and hemolymph-salivary gland interfaces, and virion circulation is expected to elicit innate defense and stress-related proteins. Our goal was to select and validate genes involved in whitefly-mediated transmission. Whitefly expressed sequence tags (ESTs) from a previous study were re-annotated, taking advantage of newly available insect EST, UniGene, and Protein sequences. Six whitefly genes and transcripts, actin, cyclophilin, GBLP, GAPDH 3, knottin, and whitefly endosymbiont HSP60, representing three gene ontology (GO) categories, were analyzed using PCR or RT- PCR, respectively, followed by cloning and DNA sequencing. Analysis confirmed the presence of all six whitefly genes and five transcripts, with the knottin transcript being undetectable.
Type:
text; Electronic Thesis
Keywords:
Bioinformatics; Defense genes; Geminivirus; Stress genes; Whitefly
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Plant Science; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Brown, Judith K.
Committee Chair:
Brown, Judith K.

Full metadata record

DC FieldValue Language
dc.language.isoENen_US
dc.titleANNOTATION OF WHITEFLY EXPRESSED SEQUENCE TAGS AND VALIDATION OF GENES WITH POTENTIAL SIGNIFICANCE TO BEGOMOVIRUS TRANSMISSIONen_US
dc.creatorSaripalli, Chandrasekharen_US
dc.contributor.authorSaripalli, Chandrasekharen_US
dc.date.issued2008en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe whitefly Bemisia tabaci (Gennadius) (Hemiptera) complex is the sole arthropod vector of the genus, Begomovirus (family, Geminiviridae), which causes debilitating diseases of plants, worldwide. Virus-vector specificity is conferred through co-evolved, whitefly vector-viral capsid protein-protein interactions. Membrane-bound receptors are thought to facilitate virion passage across the gut-hemolymph and hemolymph-salivary gland interfaces, and virion circulation is expected to elicit innate defense and stress-related proteins. Our goal was to select and validate genes involved in whitefly-mediated transmission. Whitefly expressed sequence tags (ESTs) from a previous study were re-annotated, taking advantage of newly available insect EST, UniGene, and Protein sequences. Six whitefly genes and transcripts, actin, cyclophilin, GBLP, GAPDH 3, knottin, and whitefly endosymbiont HSP60, representing three gene ontology (GO) categories, were analyzed using PCR or RT- PCR, respectively, followed by cloning and DNA sequencing. Analysis confirmed the presence of all six whitefly genes and five transcripts, with the knottin transcript being undetectable.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
dc.subjectBioinformaticsen_US
dc.subjectDefense genesen_US
dc.subjectGeminivirusen_US
dc.subjectStress genesen_US
dc.subjectWhiteflyen_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplinePlant Scienceen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorBrown, Judith K.en_US
dc.contributor.chairBrown, Judith K.en_US
dc.contributor.committeememberXiong, Zhongguoen_US
dc.contributor.committeememberTsaprailis, Georgeen_US
dc.identifier.proquest10185en_US
dc.identifier.oclc659750765en_US
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