POROUS PHOSPHOLIPID NANOSHELL PROTECTED APTAMER SENSOR FOR URINE MERCURY DETECTION

Persistent Link:
http://hdl.handle.net/10150/193450
Title:
POROUS PHOSPHOLIPID NANOSHELL PROTECTED APTAMER SENSOR FOR URINE MERCURY DETECTION
Author:
Li, Zhen
Issue Date:
2010
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Mercury exposure has been related to neurological diseases and poisoning. Quantification of mercury in biological fluids, such as serum or urine is an important diagnostic method for mercury exposure. We have developed an aptamer-encapsulated porous phospholipid nanoshell (PPN) sensor for sensing mercury in urine using a modified 15-mer single strand DNA.1 The probe is protected from DNAse and other biofouling species by encapsulation within the porous liposomes composed of mixed phospholipids, allowing direct application of the aptamer in biological fluids containing DNAse and other biofouling materials. The encapsulated sensor was directly tested in urine samples at physiological pH. We were able to detect below 100 ppb (500 nM) Hg2+ in urine (urine mercury threshold set by Biologischer Arbeitstoff Toleranz Wert or BAT)1 with no sample preparation other than pH adjustment. These results suggest that porous phospholipid nanoshells (PPNs) can serve as a general-purpose protection scaffold for biological sensing.
Type:
text; Electronic Thesis
Keywords:
aptamer; mercury; phospholipids; sensor; vesicles
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Chemistry; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Aspinwall, Craig A
Committee Chair:
Aspinwall, Craig A

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titlePOROUS PHOSPHOLIPID NANOSHELL PROTECTED APTAMER SENSOR FOR URINE MERCURY DETECTIONen_US
dc.creatorLi, Zhenen_US
dc.contributor.authorLi, Zhenen_US
dc.date.issued2010en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractMercury exposure has been related to neurological diseases and poisoning. Quantification of mercury in biological fluids, such as serum or urine is an important diagnostic method for mercury exposure. We have developed an aptamer-encapsulated porous phospholipid nanoshell (PPN) sensor for sensing mercury in urine using a modified 15-mer single strand DNA.1 The probe is protected from DNAse and other biofouling species by encapsulation within the porous liposomes composed of mixed phospholipids, allowing direct application of the aptamer in biological fluids containing DNAse and other biofouling materials. The encapsulated sensor was directly tested in urine samples at physiological pH. We were able to detect below 100 ppb (500 nM) Hg2+ in urine (urine mercury threshold set by Biologischer Arbeitstoff Toleranz Wert or BAT)1 with no sample preparation other than pH adjustment. These results suggest that porous phospholipid nanoshells (PPNs) can serve as a general-purpose protection scaffold for biological sensing.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
dc.subjectaptameren_US
dc.subjectmercuryen_US
dc.subjectphospholipidsen_US
dc.subjectsensoren_US
dc.subjectvesiclesen_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineChemistryen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorAspinwall, Craig Aen_US
dc.contributor.chairAspinwall, Craig Aen_US
dc.contributor.committeememberMcGrath, Dominic Ven_US
dc.contributor.committeememberSaavedra, Steven Sen_US
dc.identifier.proquest11312en_US
dc.identifier.oclc752261160en_US
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