Purification, Solubilization, and Characterization of Mus Musculus Left Ventricular Collagen by Electrospray Mass Spectrometry

Persistent Link:
http://hdl.handle.net/10150/193444
Title:
Purification, Solubilization, and Characterization of Mus Musculus Left Ventricular Collagen by Electrospray Mass Spectrometry
Author:
Black, Timothy James
Issue Date:
2009
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
A proteomic procedure for analyzing mouse left ventricular collagen by mass spectrometry has been developed. The procedure involves a purification step that removes non-collagenous cellular components from the collagen extracellular matrix, a step that solubilizes the collagen in aqueous solvents before it is proteolytically digested for analysis with ESI-LCMS/MS. Collagen from healthy and lathrytic mice has been positively identified by applying the SEQUEST database search algorithm to spectra from the collagen prepared using this procedure. Analysis shows that the relative percentage of collagen peptides detected in lathrytic tissue is significantly greater than that of the healthy tissue. These preliminary results suggest that the percentage of cross-linked collagen is lower in the lathrytic tissue as indicated by the greater protein sequence coverage obtained for this tissue. This procedure lays the ground work for future experimentation that has the ability to allow for the identification and quantification of cross-linked peptides.
Type:
text; Electronic Thesis
Keywords:
collagen; cross-links; electrospray; left ventricle; mass spectrometry; proteomics
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Chemistry; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Wysocki, Vicki H.
Committee Chair:
Wysocki, Vicki H.

Full metadata record

DC FieldValue Language
dc.language.isoENen_US
dc.titlePurification, Solubilization, and Characterization of Mus Musculus Left Ventricular Collagen by Electrospray Mass Spectrometryen_US
dc.creatorBlack, Timothy Jamesen_US
dc.contributor.authorBlack, Timothy Jamesen_US
dc.date.issued2009en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractA proteomic procedure for analyzing mouse left ventricular collagen by mass spectrometry has been developed. The procedure involves a purification step that removes non-collagenous cellular components from the collagen extracellular matrix, a step that solubilizes the collagen in aqueous solvents before it is proteolytically digested for analysis with ESI-LCMS/MS. Collagen from healthy and lathrytic mice has been positively identified by applying the SEQUEST database search algorithm to spectra from the collagen prepared using this procedure. Analysis shows that the relative percentage of collagen peptides detected in lathrytic tissue is significantly greater than that of the healthy tissue. These preliminary results suggest that the percentage of cross-linked collagen is lower in the lathrytic tissue as indicated by the greater protein sequence coverage obtained for this tissue. This procedure lays the ground work for future experimentation that has the ability to allow for the identification and quantification of cross-linked peptides.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
dc.subjectcollagenen_US
dc.subjectcross-linksen_US
dc.subjectelectrosprayen_US
dc.subjectleft ventricleen_US
dc.subjectmass spectrometryen_US
dc.subjectproteomicsen_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineChemistryen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorWysocki, Vicki H.en_US
dc.contributor.chairWysocki, Vicki H.en_US
dc.contributor.committeememberWirth, Mary J.en_US
dc.contributor.committeememberOlenyuk, Bogdan Z.en_US
dc.identifier.proquest10505en_US
dc.identifier.oclc659752223en_US
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