Persistent Link:
http://hdl.handle.net/10150/193385
Title:
Ferritin Secretion in Aedes aegypti Larval Cells
Author:
Shen, Meng-Chieh
Issue Date:
2006
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Female mosquitoes lay eggs after the consuming a blood meal. The iron storage protein ferritin could be involved with iron movement among body tissues in insects. Mosquito ferritin is present in hemolymph (blood) and the messages for the heavy and light chain subunits contain secretion signal sequences. These subunits may be targeted to the secretory pathway. We found that Aedes aegypti larval cells (CCL-125) exposed to iron as ferric ammonium citrate (FAC) increased ferritin secretion in a dose-dependent manner. In order to study the secretory pathway of ferritin, we attempted to disrupt the Golgi by treating CCL-125 cells with brefeldin A (BFA) and monensin. Unexpectedly, neither BFA nor monensin inhibits iron-induced ferritin secretion. These data suggest that either CCL-125 cells are highly resistant to these agents or ferritin is secreted independently of the classical ER-Golgi pathway.
Type:
text; Electronic Thesis
Keywords:
Iron; Ferritin; Secretion; Mosquito
Degree Name:
MS
Degree Level:
masters
Degree Program:
Nutritional Sciences; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Winzerling, Joy J.
Committee Chair:
Winzerling, Joy J.

Full metadata record

DC FieldValue Language
dc.language.isoENen_US
dc.titleFerritin Secretion in Aedes aegypti Larval Cellsen_US
dc.creatorShen, Meng-Chiehen_US
dc.contributor.authorShen, Meng-Chiehen_US
dc.date.issued2006en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractFemale mosquitoes lay eggs after the consuming a blood meal. The iron storage protein ferritin could be involved with iron movement among body tissues in insects. Mosquito ferritin is present in hemolymph (blood) and the messages for the heavy and light chain subunits contain secretion signal sequences. These subunits may be targeted to the secretory pathway. We found that Aedes aegypti larval cells (CCL-125) exposed to iron as ferric ammonium citrate (FAC) increased ferritin secretion in a dose-dependent manner. In order to study the secretory pathway of ferritin, we attempted to disrupt the Golgi by treating CCL-125 cells with brefeldin A (BFA) and monensin. Unexpectedly, neither BFA nor monensin inhibits iron-induced ferritin secretion. These data suggest that either CCL-125 cells are highly resistant to these agents or ferritin is secreted independently of the classical ER-Golgi pathway.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
dc.subjectIronen_US
dc.subjectFerritinen_US
dc.subjectSecretionen_US
dc.subjectMosquitoen_US
thesis.degree.nameMSen_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineNutritional Sciencesen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorWinzerling, Joy J.en_US
dc.contributor.chairWinzerling, Joy J.en_US
dc.identifier.proquest1621en_US
dc.identifier.oclc137356127en_US
All Items in UA Campus Repository are protected by copyright, with all rights reserved, unless otherwise indicated.