Development and Validation of a Novel Assay to Quantify D-Limonene in Human Adipose Biopsies

Persistent Link:
http://hdl.handle.net/10150/193375
Title:
Development and Validation of a Novel Assay to Quantify D-Limonene in Human Adipose Biopsies
Author:
Miller, Jessica Anne
Issue Date:
2007
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
d-Limonene is a lipid-soluble bioactive food component found in citrus peel. It has demonstrated strong chemopreventive effects in rodent mammary, gastric, skin, liver, and lung cancers and is correlated with a significantly reduced risk for human squamous cell skin carcinoma. Because d-limonene is fat-soluble, it may have enhanced chemopreventive activity in fatty tissues such as breast. No previous methodology to quantify d-limonene in the adipose had been developed which significantly limited d-limonene tissue distribution research. For this research, an assay to extract d-limonene from adipose tissue and quantify with GC/MS was developed and validated. Linear calibration curves were established over the range of 0.0-2,526 ng d-limonene. Extraction recovery was 80%. Satisfactory within day precision (RSD 6.75 to 9.56%) and accuracy (% difference of 2-4%) were achieved. This sensitive, accurate, and precise assay to quantify d-limonene in adipose can be used for future human or animal fatty tissue deposition studies.
Type:
text; Electronic Thesis
Keywords:
d-limonene; adipose; biomarkers; GC/MS
Degree Name:
MS
Degree Level:
masters
Degree Program:
Nutritional Sciences; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Thomson, Cynthia
Committee Chair:
Thomson, Cynthia

Full metadata record

DC FieldValue Language
dc.language.isoENen_US
dc.titleDevelopment and Validation of a Novel Assay to Quantify D-Limonene in Human Adipose Biopsiesen_US
dc.creatorMiller, Jessica Anneen_US
dc.contributor.authorMiller, Jessica Anneen_US
dc.date.issued2007en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractd-Limonene is a lipid-soluble bioactive food component found in citrus peel. It has demonstrated strong chemopreventive effects in rodent mammary, gastric, skin, liver, and lung cancers and is correlated with a significantly reduced risk for human squamous cell skin carcinoma. Because d-limonene is fat-soluble, it may have enhanced chemopreventive activity in fatty tissues such as breast. No previous methodology to quantify d-limonene in the adipose had been developed which significantly limited d-limonene tissue distribution research. For this research, an assay to extract d-limonene from adipose tissue and quantify with GC/MS was developed and validated. Linear calibration curves were established over the range of 0.0-2,526 ng d-limonene. Extraction recovery was 80%. Satisfactory within day precision (RSD 6.75 to 9.56%) and accuracy (% difference of 2-4%) were achieved. This sensitive, accurate, and precise assay to quantify d-limonene in adipose can be used for future human or animal fatty tissue deposition studies.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
dc.subjectd-limoneneen_US
dc.subjectadiposeen_US
dc.subjectbiomarkersen_US
dc.subjectGC/MSen_US
thesis.degree.nameMSen_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineNutritional Sciencesen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorThomson, Cynthiaen_US
dc.contributor.chairThomson, Cynthiaen_US
dc.identifier.proquest2537en_US
dc.identifier.oclc659748471en_US
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