Silica Colloidal Crystals as Porous Substrates for Total Internal Reflection Fluorescence Microscopy

Persistent Link:
http://hdl.handle.net/10150/193371
Title:
Silica Colloidal Crystals as Porous Substrates for Total Internal Reflection Fluorescence Microscopy
Author:
Bethea, Tomika R. C.
Issue Date:
2006
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
In cell biology and chemistry, total internal reflection microscopy (TIRFM) has proven to be a useful technique that allows the probing of cellular processes with high-signal-to-noise ratio imaging. However, samples on solid substrates limit the accessibility to probe processes on extracellular membrane surface closest to the microscope objective. Colloidal crystals provide a porous alternative to the traditional solid substrates. Thin crystals exhibit optical properties similar to that of a fused silica coverslip allowing for TIRFM in the same manner as with a typical coverslip as demonstrated by the observance of Chinese hamster ovary cells with fluorescently labeled receptors on both types of substrates. Accessibility of the cell membrane closest to the substrate and the ability to probe fluorophore orientation information was observed by the binding of TIPP-cy5 to the human delta opioid receptor.
Type:
text; Electronic Thesis
Keywords:
silica; TIRF; porous substrate; colloid; membrane; crystal
Degree Name:
MS
Degree Level:
masters
Degree Program:
Chemistry; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Wirth, Mary J
Committee Chair:
Wirth, Mary J

Full metadata record

DC FieldValue Language
dc.language.isoENen_US
dc.titleSilica Colloidal Crystals as Porous Substrates for Total Internal Reflection Fluorescence Microscopyen_US
dc.creatorBethea, Tomika R. C.en_US
dc.contributor.authorBethea, Tomika R. C.en_US
dc.date.issued2006en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractIn cell biology and chemistry, total internal reflection microscopy (TIRFM) has proven to be a useful technique that allows the probing of cellular processes with high-signal-to-noise ratio imaging. However, samples on solid substrates limit the accessibility to probe processes on extracellular membrane surface closest to the microscope objective. Colloidal crystals provide a porous alternative to the traditional solid substrates. Thin crystals exhibit optical properties similar to that of a fused silica coverslip allowing for TIRFM in the same manner as with a typical coverslip as demonstrated by the observance of Chinese hamster ovary cells with fluorescently labeled receptors on both types of substrates. Accessibility of the cell membrane closest to the substrate and the ability to probe fluorophore orientation information was observed by the binding of TIPP-cy5 to the human delta opioid receptor.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
dc.subjectsilicaen_US
dc.subjectTIRFen_US
dc.subjectporous substrateen_US
dc.subjectcolloiden_US
dc.subjectmembraneen_US
dc.subjectcrystalen_US
thesis.degree.nameMSen_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineChemistryen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorWirth, Mary Jen_US
dc.contributor.chairWirth, Mary Jen_US
dc.identifier.proquest1985en_US
dc.identifier.oclc659746556en_US
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