Persistent Link:
http://hdl.handle.net/10150/193356
Title:
Epigenetic Effects of Arsenite in HeLa Cells
Author:
Burgos, Rosa M
Issue Date:
2007
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Mechanisms of arsenic toxicity are not yet clear. Arsenite has effects on methylation pathways, by decreasing expression of DNA methylases and depletion of S-adenosylmethionine. Histones are DNA packing proteins that regulate gene expression modulating chromatin accessibility. Methylation at Lysine 9 of Histone H3 (K9H3) is a hallmark of heterochromatin. Dimethyl K9H3 is a mark of facultative heterochromatin and trimethyl K9H3 is present on constitutive heterochromatin. HeLa cells exposed for 24 hrs to 1 uM or 5 uM Sodium Arsenite were fixed and different posttranslational modifications of histones were detected by indirect immunofluorescence. Images were analyzed to assess the change on average methylated species of K9H3 in cell nuclei. Interestingly Arsenite (1 uM and 5 uM) treated cells had a significant increase in the trimethylated and dimethylated of K9H3, evaluated throught the comparison of average nuclei brightness and pixel value analysis between treatments.
Type:
text; Electronic Thesis
Keywords:
histone; arsenic; methylation; epigenetic; H3
Degree Name:
MS
Degree Level:
masters
Degree Program:
Molecular & Cellular Biology; Graduate College
Degree Grantor:
University of Arizona
Committee Chair:
Dixon, Kathleen

Full metadata record

DC FieldValue Language
dc.language.isoENen_US
dc.titleEpigenetic Effects of Arsenite in HeLa Cellsen_US
dc.creatorBurgos, Rosa Men_US
dc.contributor.authorBurgos, Rosa Men_US
dc.date.issued2007en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractMechanisms of arsenic toxicity are not yet clear. Arsenite has effects on methylation pathways, by decreasing expression of DNA methylases and depletion of S-adenosylmethionine. Histones are DNA packing proteins that regulate gene expression modulating chromatin accessibility. Methylation at Lysine 9 of Histone H3 (K9H3) is a hallmark of heterochromatin. Dimethyl K9H3 is a mark of facultative heterochromatin and trimethyl K9H3 is present on constitutive heterochromatin. HeLa cells exposed for 24 hrs to 1 uM or 5 uM Sodium Arsenite were fixed and different posttranslational modifications of histones were detected by indirect immunofluorescence. Images were analyzed to assess the change on average methylated species of K9H3 in cell nuclei. Interestingly Arsenite (1 uM and 5 uM) treated cells had a significant increase in the trimethylated and dimethylated of K9H3, evaluated throught the comparison of average nuclei brightness and pixel value analysis between treatments.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
dc.subjecthistoneen_US
dc.subjectarsenicen_US
dc.subjectmethylationen_US
dc.subjectepigeneticen_US
dc.subjectH3en_US
thesis.degree.nameMSen_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineMolecular & Cellular Biologyen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.chairDixon, Kathleenen_US
dc.identifier.proquest2207en_US
dc.identifier.oclc659748441en_US
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