The Effect of Lithium Chloride on the Distal Insulin Signaling Cascade and on p38 MAPK in the Soleus Muscle of Female Lean Zucker Rats

Persistent Link:
http://hdl.handle.net/10150/193350
Title:
The Effect of Lithium Chloride on the Distal Insulin Signaling Cascade and on p38 MAPK in the Soleus Muscle of Female Lean Zucker Rats
Author:
Gifford, Nancy Renee
Issue Date:
2007
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
This project focused on determining the effect of lithium on glucose uptake, glycogen synthesis, and insulin signaling proteins, protein kinase B (Akt1) and GSK-3, in isolated soleus muscle from female lean Zucker rats. We also investigated the role of the stress-activated p38 MAPK in the action of lithium to activate skeletal muscle glucose transport. In the absence of insulin, lithium (10 mM LiCl) increased basal glucose transport by 62% (p<0.05) and glycogen synthesis by 112%. Lithium did not alter phosphorylation of Akt ser473, but enhanced GSK-3β ser9 phosphorylation by 41%. Lithium further enhanced the effect of insulin on glucose transport (42%), glycogen synthesis (44%), and GSK-3ß phosphorylation (13%). Lithium increased phosphorylated p38 MAPK 31% without and 19% with insulin. Moreover, a selective p38 MAPK inhibitor, A304000, completely prevented the lithium-induced enhancement of glucose transport revealing the critical involvement of p38 MAPK phosphorylation in lithium-induced glucose transport in isolated skeletal muscle.
Type:
text; Electronic Thesis
Keywords:
Insulin signaling cascade; lithium; p38 MAPK
Degree Name:
MS
Degree Level:
masters
Degree Program:
Biochemistry; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Henriksen, Erik J.
Committee Chair:
Henriksen, Erik J.

Full metadata record

DC FieldValue Language
dc.language.isoENen_US
dc.titleThe Effect of Lithium Chloride on the Distal Insulin Signaling Cascade and on p38 MAPK in the Soleus Muscle of Female Lean Zucker Ratsen_US
dc.creatorGifford, Nancy Reneeen_US
dc.contributor.authorGifford, Nancy Reneeen_US
dc.date.issued2007en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThis project focused on determining the effect of lithium on glucose uptake, glycogen synthesis, and insulin signaling proteins, protein kinase B (Akt1) and GSK-3, in isolated soleus muscle from female lean Zucker rats. We also investigated the role of the stress-activated p38 MAPK in the action of lithium to activate skeletal muscle glucose transport. In the absence of insulin, lithium (10 mM LiCl) increased basal glucose transport by 62% (p<0.05) and glycogen synthesis by 112%. Lithium did not alter phosphorylation of Akt ser473, but enhanced GSK-3β ser9 phosphorylation by 41%. Lithium further enhanced the effect of insulin on glucose transport (42%), glycogen synthesis (44%), and GSK-3ß phosphorylation (13%). Lithium increased phosphorylated p38 MAPK 31% without and 19% with insulin. Moreover, a selective p38 MAPK inhibitor, A304000, completely prevented the lithium-induced enhancement of glucose transport revealing the critical involvement of p38 MAPK phosphorylation in lithium-induced glucose transport in isolated skeletal muscle.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
dc.subjectInsulin signaling cascadeen_US
dc.subjectlithiumen_US
dc.subjectp38 MAPKen_US
thesis.degree.nameMSen_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineBiochemistryen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorHenriksen, Erik J.en_US
dc.contributor.chairHenriksen, Erik J.en_US
dc.identifier.proquest2135en_US
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