Characterization of Membrane Permeability and Polymer-Stabilized Model Membranes

Persistent Link:
http://hdl.handle.net/10150/193347
Title:
Characterization of Membrane Permeability and Polymer-Stabilized Model Membranes
Author:
Ma, Yaning
Issue Date:
2007
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The permeability of lipid bilayer membranes to glucose and carboxyfluorescein has been studied in model membranes. Using an enzyme assay, the permeability of glucose was monitored spectrometrically with both large and giant unilamellar vesicles (LUVs and GUVs). The permeability of carboxyfluorescein was studied by entrapping the dye and monitoring its leakage over time from a single GUV. Permeability study using GUVs may provide new information that cannot be obtained from LUVs.The stability of lipid membranes was enhanced by incorporating polymer scaffold. LUVs were prepared with hydrophobic monomers partitioned and then polymerized inside the hydrophobic interior of the lipid bilayers. The sizes of the formed polymers were characterized using gel permeation chromatography and mass spectrometry. This study suggests that large molecular weight polymers were formed inside the lipid bilayers and that the stability of the membranes is related to the size of the polymers.
Type:
text; Electronic Thesis
Keywords:
Giant unilamellar vesicle; Epi-fluorescence; Polymer; Mass spectrometry; Gel permeation chromatography
Degree Name:
MS
Degree Level:
masters
Degree Program:
Chemistry; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Saavedra, Steve Scott
Committee Chair:
Saavedra, Steve Scott

Full metadata record

DC FieldValue Language
dc.language.isoENen_US
dc.titleCharacterization of Membrane Permeability and Polymer-Stabilized Model Membranesen_US
dc.creatorMa, Yaningen_US
dc.contributor.authorMa, Yaningen_US
dc.date.issued2007en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe permeability of lipid bilayer membranes to glucose and carboxyfluorescein has been studied in model membranes. Using an enzyme assay, the permeability of glucose was monitored spectrometrically with both large and giant unilamellar vesicles (LUVs and GUVs). The permeability of carboxyfluorescein was studied by entrapping the dye and monitoring its leakage over time from a single GUV. Permeability study using GUVs may provide new information that cannot be obtained from LUVs.The stability of lipid membranes was enhanced by incorporating polymer scaffold. LUVs were prepared with hydrophobic monomers partitioned and then polymerized inside the hydrophobic interior of the lipid bilayers. The sizes of the formed polymers were characterized using gel permeation chromatography and mass spectrometry. This study suggests that large molecular weight polymers were formed inside the lipid bilayers and that the stability of the membranes is related to the size of the polymers.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
dc.subjectGiant unilamellar vesicleen_US
dc.subjectEpi-fluorescenceen_US
dc.subjectPolymeren_US
dc.subjectMass spectrometryen_US
dc.subjectGel permeation chromatographyen_US
thesis.degree.nameMSen_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineChemistryen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorSaavedra, Steve Scotten_US
dc.contributor.chairSaavedra, Steve Scotten_US
dc.identifier.proquest2384en_US
dc.identifier.oclc659748274en_US
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