Small Molecule Matrix-free Laser Desorption/ionization Mass Spectrometry

Persistent Link:
http://hdl.handle.net/10150/193332
Title:
Small Molecule Matrix-free Laser Desorption/ionization Mass Spectrometry
Author:
Wen, Xiujuan
Issue Date:
2006
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Modified Si wafers and nanoparticles (5-50 nm) have been developed and explored to assist laser desorption ionization mass spectrometry (LDI-MS) for small molecule analysis. DIOS (desorption/ionization on silicon) plates were prepared according to published protocols. DIOS was tested and compared with the optimized silicon nanoparticles derivatized by penterfluorophenylchlorosilane (PFP). SPALDI (Si nanoparticle assisted LDI) requires less laser flux than common MALDI and DIOS thus provides significant less background and higher ionization efficiency. It has higher surface homogeneity, relative salt tolerance and high selectivity which may origin from analyte dependent pre-charging. Surface characterization has been investigated. And different analytes including drugs, peptides, pesticides and acids in both biological and environmental samples have been applied by positive or negative ionization mode. Detection limits, down to the low femtomole per microliter levels have been achieved for propafenone and verapamil. SPALDI is an easily applicable practical tool at a potential low cost.
Type:
text; Electronic Thesis
Keywords:
Mass Spectrometry
Degree Name:
MS
Degree Level:
masters
Degree Program:
Chemistry; Graduate College
Degree Grantor:
University of Arizona
Committee Chair:
Wysocki, Vicki H.

Full metadata record

DC FieldValue Language
dc.language.isoENen_US
dc.titleSmall Molecule Matrix-free Laser Desorption/ionization Mass Spectrometryen_US
dc.creatorWen, Xiujuanen_US
dc.contributor.authorWen, Xiujuanen_US
dc.date.issued2006en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractModified Si wafers and nanoparticles (5-50 nm) have been developed and explored to assist laser desorption ionization mass spectrometry (LDI-MS) for small molecule analysis. DIOS (desorption/ionization on silicon) plates were prepared according to published protocols. DIOS was tested and compared with the optimized silicon nanoparticles derivatized by penterfluorophenylchlorosilane (PFP). SPALDI (Si nanoparticle assisted LDI) requires less laser flux than common MALDI and DIOS thus provides significant less background and higher ionization efficiency. It has higher surface homogeneity, relative salt tolerance and high selectivity which may origin from analyte dependent pre-charging. Surface characterization has been investigated. And different analytes including drugs, peptides, pesticides and acids in both biological and environmental samples have been applied by positive or negative ionization mode. Detection limits, down to the low femtomole per microliter levels have been achieved for propafenone and verapamil. SPALDI is an easily applicable practical tool at a potential low cost.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
dc.subjectMass Spectrometryen_US
thesis.degree.nameMSen_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineChemistryen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.chairWysocki, Vicki H.en_US
dc.identifier.proquest1643en_US
dc.identifier.oclc137356209en_US
All Items in UA Campus Repository are protected by copyright, with all rights reserved, unless otherwise indicated.