Persistent Link:
http://hdl.handle.net/10150/193318
Title:
Interaction of Bovine Seminal Proteins with Neutrophils
Author:
Cropp, Amy Rena
Issue Date:
2006
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Neutrophils ordinarily infiltrate the female reproductive tract subsequent to mating or artificial insemination, resulting in reduced fertility. Recently, it was demonstrated that equine neutrophil extracellular traps (NETs) entangled sperm in these DNA-rich structures, interfering with their normal transport through the female reproductive tract. Seminal plasma (SP) or proteinaceous extracts from SP inhibited sperm-neutrophil binding and specifically degraded sperm-activated NETs, without suppressing bactericidal activity of neutrophils. Fertility-associated antigen (FAA), a 31 kDa naturally occurring heparin-binding protein (HBP) produced by the accessory sex glands, has been shown to bind to sperm and potentiate heparin-induced capacitation. FAA shares 87% identity with DNase I-like family members, and contains two internal DNase-I-like peptide motifs. The purpose of this study was to determine if a recombinant form of FAA displayed capacitating effects associated with the native protein and to determine whether rFAA displayed DNase activity similar to SP or SP protein extracts to inhibit sperm-neutrophil binding.
Type:
text; Electronic Thesis
Keywords:
Semen; Neutrophil; Fertility; Recombinant Protein; Sperm
Degree Name:
MS
Degree Level:
masters
Degree Program:
Animal Sciences; Graduate College
Degree Grantor:
University of Arizona
Advisor:
Ax, Roy L.
Committee Chair:
Ax, Roy L.

Full metadata record

DC FieldValue Language
dc.language.isoENen_US
dc.titleInteraction of Bovine Seminal Proteins with Neutrophilsen_US
dc.creatorCropp, Amy Renaen_US
dc.contributor.authorCropp, Amy Renaen_US
dc.date.issued2006en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractNeutrophils ordinarily infiltrate the female reproductive tract subsequent to mating or artificial insemination, resulting in reduced fertility. Recently, it was demonstrated that equine neutrophil extracellular traps (NETs) entangled sperm in these DNA-rich structures, interfering with their normal transport through the female reproductive tract. Seminal plasma (SP) or proteinaceous extracts from SP inhibited sperm-neutrophil binding and specifically degraded sperm-activated NETs, without suppressing bactericidal activity of neutrophils. Fertility-associated antigen (FAA), a 31 kDa naturally occurring heparin-binding protein (HBP) produced by the accessory sex glands, has been shown to bind to sperm and potentiate heparin-induced capacitation. FAA shares 87% identity with DNase I-like family members, and contains two internal DNase-I-like peptide motifs. The purpose of this study was to determine if a recombinant form of FAA displayed capacitating effects associated with the native protein and to determine whether rFAA displayed DNase activity similar to SP or SP protein extracts to inhibit sperm-neutrophil binding.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
dc.subjectSemenen_US
dc.subjectNeutrophilen_US
dc.subjectFertilityen_US
dc.subjectRecombinant Proteinen_US
dc.subjectSpermen_US
thesis.degree.nameMSen_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineAnimal Sciencesen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.advisorAx, Roy L.en_US
dc.contributor.chairAx, Roy L.en_US
dc.contributor.committeememberReggiardo, Carlosen_US
dc.contributor.committeememberLimesand, Seanen_US
dc.identifier.proquest1920en_US
dc.identifier.oclc659746485en_US
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