THE INFLUENCE OF CHOLESTEROL LOADING AND SUBSEQUENT UNLOADING IN PRESERVATION OF STALLION SPERMATOZOA

Persistent Link:
http://hdl.handle.net/10150/193308
Title:
THE INFLUENCE OF CHOLESTEROL LOADING AND SUBSEQUENT UNLOADING IN PRESERVATION OF STALLION SPERMATOZOA
Author:
Anderson, Crystal R.
Issue Date:
2005
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The influences of loading cholesterol into stallion spermatozoa membranes prior to cold storage or cryopreservation were determined using cholesterol loaded cyclodextrin (CLC) before preservation, followed by the unloading of cholesterol after preservation using methyl beta cyclodextrin (MBCD). Experiment I: dose response trials determining optimal amounts of CLC and MBCD based on percentages of progressively motile spermatozoa (PMS) following preservation. Experiment II: influences of CLC and MBCD on PMS, the percentages of live intact (LI) and live non-intact (LNI) spermatozoa following cold storage. Experiment III: influences of CLC before cryopreservation and MBCD on PMS, LI, and LNI post-thaw. Addition of CLC improved (P<0.05) PMS and LI following preservation when compared to the control. Unloading cholesterol using MBCD does not alter PMS, LI nor LNI. Addition of CLC is beneficial to survival of spermatozoa following preservation and addition of MBCD in small amounts does not negatively influence PMS, LI or LNI.
Type:
text; Electronic Thesis
Keywords:
Equine; Cyclodextrin; Cryopreservation; Cold Storage; Cholesterol; Spermatozoa
Degree Name:
MS
Degree Level:
masters
Degree Program:
Animal Sciences; Graduate College
Degree Grantor:
University of Arizona
Committee Chair:
Arns, Mark J

Full metadata record

DC FieldValue Language
dc.language.isoENen_US
dc.titleTHE INFLUENCE OF CHOLESTEROL LOADING AND SUBSEQUENT UNLOADING IN PRESERVATION OF STALLION SPERMATOZOAen_US
dc.creatorAnderson, Crystal R.en_US
dc.contributor.authorAnderson, Crystal R.en_US
dc.date.issued2005en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractThe influences of loading cholesterol into stallion spermatozoa membranes prior to cold storage or cryopreservation were determined using cholesterol loaded cyclodextrin (CLC) before preservation, followed by the unloading of cholesterol after preservation using methyl beta cyclodextrin (MBCD). Experiment I: dose response trials determining optimal amounts of CLC and MBCD based on percentages of progressively motile spermatozoa (PMS) following preservation. Experiment II: influences of CLC and MBCD on PMS, the percentages of live intact (LI) and live non-intact (LNI) spermatozoa following cold storage. Experiment III: influences of CLC before cryopreservation and MBCD on PMS, LI, and LNI post-thaw. Addition of CLC improved (P<0.05) PMS and LI following preservation when compared to the control. Unloading cholesterol using MBCD does not alter PMS, LI nor LNI. Addition of CLC is beneficial to survival of spermatozoa following preservation and addition of MBCD in small amounts does not negatively influence PMS, LI or LNI.en_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
dc.subjectEquineen_US
dc.subjectCyclodextrinen_US
dc.subjectCryopreservationen_US
dc.subjectCold Storageen_US
dc.subjectCholesterolen_US
dc.subjectSpermatozoaen_US
thesis.degree.nameMSen_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineAnimal Sciencesen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.chairArns, Mark Jen_US
dc.identifier.proquest1158en_US
dc.identifier.oclc659747439en_US
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