Development and investigation of a multiparameter microbial toxicity test using the bacterium Salmonella typhimurium

Persistent Link:
http://hdl.handle.net/10150/191970
Title:
Development and investigation of a multiparameter microbial toxicity test using the bacterium Salmonella typhimurium
Author:
Kupillas, Gregory Edward,1959-
Issue Date:
1988
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Experimental procedures were developed to test the effects of various pure compounds and papermill wastewater samples on the growth rate, intracellular ATP concentration and oxygen-utilization rate of the bacterium, Salmonella typhimurium TA98. Responses of the bacterium to increasing concentrations of three specific inhibitors, cyanide, 2,4-dinitrophenol, and chloramphenicol corresponded well with the predicted results. Comparison of microbial responses to specific inhibitors and mechanistically uncharacterized compounds suggested general inhibitory mechanisms for the latter chemicals. Correlations between measured relative toxicities of a homologous series of chlorinated phenols with the log octanol/water partition coefficients of those compounds indicated a strong relationship between toxicity and hydrophobicity. Correlations with published toxicity data for the pure compounds showed generally good agreement between the S. typhimurium test and other aquatic toxicity tests. Intracellular ATP concentration was the most sensitive indicator of toxicity in tests involving papermill wastewater samples.
Type:
Thesis-Reproduction (electronic); text
LCSH Subjects:
Hydrology.; Microbiological assay.; Water -- Pollution -- Toxicology.; Salmonella typhimurium.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Hydrology and Water Resources; Graduate College
Degree Grantor:
University of Arizona
Committee Chair:
Arnold, R. G.; Bales, R. C.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleDevelopment and investigation of a multiparameter microbial toxicity test using the bacterium Salmonella typhimuriumen_US
dc.creatorKupillas, Gregory Edward,1959-en_US
dc.contributor.authorKupillas, Gregory Edward,1959-en_US
dc.date.issued1988en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractExperimental procedures were developed to test the effects of various pure compounds and papermill wastewater samples on the growth rate, intracellular ATP concentration and oxygen-utilization rate of the bacterium, Salmonella typhimurium TA98. Responses of the bacterium to increasing concentrations of three specific inhibitors, cyanide, 2,4-dinitrophenol, and chloramphenicol corresponded well with the predicted results. Comparison of microbial responses to specific inhibitors and mechanistically uncharacterized compounds suggested general inhibitory mechanisms for the latter chemicals. Correlations between measured relative toxicities of a homologous series of chlorinated phenols with the log octanol/water partition coefficients of those compounds indicated a strong relationship between toxicity and hydrophobicity. Correlations with published toxicity data for the pure compounds showed generally good agreement between the S. typhimurium test and other aquatic toxicity tests. Intracellular ATP concentration was the most sensitive indicator of toxicity in tests involving papermill wastewater samples.en_US
dc.description.notehydrology collectionen_US
dc.typeThesis-Reproduction (electronic)en_US
dc.typetexten_US
dc.subject.lcshHydrology.en_US
dc.subject.lcshMicrobiological assay.en_US
dc.subject.lcshWater -- Pollution -- Toxicology.en_US
dc.subject.lcshSalmonella typhimurium.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineHydrology and Water Resourcesen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.chairArnold, R. G.en_US
dc.contributor.chairBales, R. C.en_US
dc.identifier.oclc213332748en_US
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