Persistent Link:
http://hdl.handle.net/10150/191916
Title:
Detection of Cryptosporidium and Giardia in environmental waters
Author:
Sullivan-Madore, Mary,1959-
Issue Date:
1986
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Cryptosporidium and Giardia, both enteric parasites, have been shown to cause waterborne disease. Methods were developed to investigate the occurrence of Cryptosporidium and Giardia in large volumes of water. Environmental waters were filtered, eluted from a filter and concentrated using centrifugation. Antifoam was employed after homogenization of the resultant pellet suspension for faster oocyst recoveries. This suspension was then layered onto a density gradient to separate the parasites from the sediment. The gradient layer containing any potential parasites was directly labeled on the membrane filter with fluorescein labeled monoclonal antibodies. These filters were then examined by immunofluorescence. Using this technique, Cryptosporidium oocysts were detected in raw sewage, treated sewage, backflush waters, and surface waters. Cryptosporidium was three magnitudes higher in concentration than Giardia in raw sewage. Since Cryptosporidium is frequently present in environmental waters, and in significantly higher numbers than Giardia, it potentially could be transmitted by this route.
Type:
Thesis-Reproduction (electronic); text
LCSH Subjects:
Hydrology.; Cryptosporidium.; Giardia.; Water -- Microbiology.; Waterborne infection.
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Nutrition and Food Sciences; Graduate College
Degree Grantor:
University of Arizona
Committee Chair:
Gerba, Charles P.

Full metadata record

DC FieldValue Language
dc.language.isoenen_US
dc.titleDetection of Cryptosporidium and Giardia in environmental watersen_US
dc.creatorSullivan-Madore, Mary,1959-en_US
dc.contributor.authorSullivan-Madore, Mary,1959-en_US
dc.date.issued1986en_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.description.abstractCryptosporidium and Giardia, both enteric parasites, have been shown to cause waterborne disease. Methods were developed to investigate the occurrence of Cryptosporidium and Giardia in large volumes of water. Environmental waters were filtered, eluted from a filter and concentrated using centrifugation. Antifoam was employed after homogenization of the resultant pellet suspension for faster oocyst recoveries. This suspension was then layered onto a density gradient to separate the parasites from the sediment. The gradient layer containing any potential parasites was directly labeled on the membrane filter with fluorescein labeled monoclonal antibodies. These filters were then examined by immunofluorescence. Using this technique, Cryptosporidium oocysts were detected in raw sewage, treated sewage, backflush waters, and surface waters. Cryptosporidium was three magnitudes higher in concentration than Giardia in raw sewage. Since Cryptosporidium is frequently present in environmental waters, and in significantly higher numbers than Giardia, it potentially could be transmitted by this route.en_US
dc.description.notehydrology collectionen_US
dc.typeThesis-Reproduction (electronic)en_US
dc.typetexten_US
dc.subject.lcshHydrology.en_US
dc.subject.lcshCryptosporidium.en_US
dc.subject.lcshGiardia.en_US
dc.subject.lcshWater -- Microbiology.en_US
dc.subject.lcshWaterborne infection.en_US
thesis.degree.nameM.S.en_US
thesis.degree.levelmastersen_US
thesis.degree.disciplineNutrition and Food Sciencesen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.grantorUniversity of Arizonaen_US
dc.contributor.chairGerba, Charles P.en_US
dc.contributor.committeememberSterling, Charlesen_US
dc.contributor.committeememberArrowood, Michael J.en_US
dc.contributor.committeememberMead, Janen_US
dc.identifier.oclc213416626en_US
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